High Resolution Spectrometer 200-1100nm
Back-Illuminated Spectrometer 200-1100nm
Cooled Spectrometers 200-1100nm
Large NA High Sensitivity Spectrometer
Raman Spectrometer 532/785/1064nm
High-speed Line Scan Confocal Microscope
Fluorescence / PL Microscope
Time Correlated Single Photon Counting
Temperature Control Stage 77K
Maskless Lithography UV Laser Writer
Laser Doppler Vibrometer
OCT Imaging System
Terahertz TPX Lens
Off-Axis Parabolic Mirrors
780nm Femtosecond Laser
1560nm Femtosecond Laser
Fiber Spectrometers (200nm to 5um)
X-Ray/XUV/VUV Spectrometers (1-300nm)
Hyperspectral Camera (220nm-4.2um)
Multi-Spectral Camera (400-850nm)
Single Photon Counting Imager
Visible Single Photon Detector(SPD)
Infrared Single Photon Detector(SPD)
Photodiode & Photomultiplier (200nm-12um)
Infrared Linear Detector /LVF (2-12um)
Standard Beam Profiler (190-1100nm)
1inch Aperture Beam Profiler (190-1100nm)
SWIR Beam Profiler (900-1700nm)
IR Beam Profiler (2-16um)
Terahertz Beam Profiler(1-18 THz)
Scanning Slit Beam Profiler (190-2500nm)
Power Meter Sensor Head
Sensor Controller (OPM)
Laser Diode LIV Testing Systems
Adaptor & Accessories
Options and Accessories
Autocorrelator - Single Shot
Autocorrelator - Multi Shot
FROG - Single Shot
FROG - Multi Shot
Optical Test Measurement System
RF Test Measurement System
Pigtailed Laser Diode (400nm-1550nm)
CW Laser Diode Module
DPSS Pulsed Nanosecond Lasers
DFB/FP Picosecond Laser (370-1550nm)
Nanosecond Pulse Fiber Laser
Picosecond Pulse Fiber Laser
Femtosecond Pulse Fiber Laser
CW Single Wavelength Fiber Laser
Ultra-Narrow Linewidth Lasers
C-Band Tunable Laser
L-Band Tunable Laser
2000nm Widely Tuneable Laser
Supercontinuum Fiber Lasers 450-2300nm
Broadband Femtosecond Laser 950-1150nm
Erbium Doped Fiber Amplifier
Ytterbium Doped Fiber Amplifier
Thulium-Doped Fiber Amplifier
Fiber Raman Amplifier
Single Wavelength LED Source(240-980nm)
Multi-wavelength LEDs Source (240-980nm)
ASE/SLD Light Sources (850-2000nm)
IR Emitter Chip (2-14um）
Light & Wavelength Selector
Laser Safety Glasses
Light Field Sythesizer
White Light Interferometer
CRD Loss Meter and Reflectometer
Hollow-Core Fiber Compressor
High Powered Hollow-Core Fiber Compressor
Ultra-High Contrast 3rd-Order Autocorrelator
X-ray sCMOS Camera
XUV/VUV Cameras (1-350nm)
UV-NIR sCMOS Camera (200-1100nm)
Intensified CMOS Camera / Intensifier
CMOS Camera (VIS-NIR)
HDMI Color CMOS Camera
Infrared Line Array Camera
Infrared Matrix Array Cameras
Blackbody Calibration Sources
Hyperspectral Camera LineScan (0.22-4.2um)
Hyperspectral Camera SnapShot(0.35-1um)
ID900 Timing Controller
ID1000 Timing Controller
General Purpose Pulse Generators
Medium and High Voltage Pulse Generators
High Speed Impulse Generator
Very High Speed Pulse Generators
12.7mm Optical Post Assemblies
25.4mm Optical Post Assemblies
Mini-Series Optical Post Assemblies
Kinematic Mirror Mounts
Fixed Mirror Mounts
Gimbal Mirror Mounts
Piezo Mirror Mounts
Honeycomb Optical Breadboards
Tensile Micro-strain Stage
Plano Convex Spherical Lens
Plano Concave Spherical Lens
Bi-Convex Spherical Lens
Bi-Concave Spherical Lens
Dielectric Laser Line Mirrors
Broadband Dielectric Mirrors
Right Angle Prism Mirrors
Ultrafast Laser Mirrors
Right Angle Prism
Ultrathin Beamsplitter Plate
Neutral Density Filters
Category Overview (Filters)
Nonlinear Optical Crystals
AR Coated Round Windows
Laser Protection Windows
In other words, LINCam is just a camera. As easy as an ordinal megapixel CCD camera but extended with the timing dimension.
• Fluorescence lifetime imaging (FLIM)
• Light-sheet 3D FLIM• Time resolved Raman spectroscopy• Time-of-Flight measurements
• Low-light observations
Product specifications and Brochures
Product Brochure Link:
Maximal Count Rate, MHz
Confocal Superresolution FLIM Microscopy
Primary hippocampal neurons from rats.To visualize excitatory synaptic contacts,
neurons were stained with rat anti-homer, guinea pig anti-MAP2, rat anti-Ctip2
and mouse anti- Prox1 antibodies. Subsequently, samples were incubated with
anti-rat Alexa 488-, anti-guinea pig Cy5-, and anti-mouse Alexa 350- conjugated
donkey secondary antibodies.
Lymphocytes (Jurakt T-cells) were transfected with a monomeric CFP-YFP Lck-biosensor and stimulated by CD3. After fixation cells were immune stained by an anti-GFP antibody, labelled with Atto 647N. The optical sections clearly show the shuttling of Lck positive vesicle between the plasma membrane and an inner compartment (most likely associated with the Golgi complex). Intensity weighted 3D stack of FLIM images of T-cells, 400 × 400 bins, 20 seconds per slice.
Rat embryo acquired with light-sheet microscope
One of the well-known drawbacks of widefield microscopy imaging its
low axial resolution compared to confocal microscopy. Sevaral methods are
known to overcome those limitations introducing confocality. Here we
demonstrate FLIM images acquired with optical sectioning with light-sheet illumination.
A commercially available light-sheet system equipped with a pulsed laser source and a CCD with c-mount. For the user it is just a drop-in replacement of the CCD by LINCam to start imaging. An image below shows 128 FLIM sections acquired within 10 seconds per frame.
FILM (Fluorescence lifetime Imaging)
Fluorescence Lifetime Imaging (FLIM) is a technique which uses the separation of different fluorescence decay times of fluorophores to create an image contrast other than intensity in classical imaging.
Example of lifetime imaging of a lily of the valley slice sample. The intensity image
(a) is a histogram of the positions of acquired photons. Lifetime analysis reveals four
lifetime components: τ1 = 0,19; τ2 = 0,67; τ3 = 1,95 and τ4 = 3,75 ns. The resulting
(b) of the intensity image and average lifetime is shown.
Glycolytic Oscillations in Eukaryotic Cells Followed by NADH Imaging
By using the metabolite NADH as an intrinsic marker for glycolysis, the dynamics of individual cells can be monitored and their interactions studied.
Monitoring intrinsic energy metabolism over long periods of time allows the study of cellular communication between cell populations. By using the metabolite NADH as an intrinsic marker for glycolysis, the dynamics of individual cells can be monitored and their interactions studied. Glucose consumption by glycolysis and alcoholic fermentation leads to the production of metabolites, some of which are released. Coupling between yeast cells depends on the release and sensing of the messenger acetaldehyde, which diffuses through the extracellular medium. Yeast cells are well known for the oscillatory behavior of the glycolysis and their metabolic organization. The exchange of messenger molecules can result in waves and synchronized patterns in which all cells oscillate in concert. Essential to this study is an ultrasensitive detection system that allows excitation of the weak fluorescence of NADH by low-intensity UV light.
(a) The time-series of the collective NADH fluorescence signal for a yeast population of cell density ρ=0.1%. Partial synchronisation of intracellular oscillations occurs at
760 s ≤t≤1100 s. (b) Development of the relative amplitudes of oscillations of each cell, and (c) of their phases. In (b) and (c) the cells are sorted according to their phases at time t=900 s. (d) Evolution of the distribution of instantaneous frequencies fi of the cells, and (e) of the distribution of the phase difference Δϕi between the phase ϕi of each individual cell to that of the average phase Φ of all cells of the population. (f) Time dependence of the order parameter R. The field of view had a diameter of 169 μm, and hosted 232 cells. Glucose was added to the cell suspension at t=−158 s.
Measurement induced entanglement of stored ions
Typically single photon emitters show an emission behaviour that is called anti-bunching. This means that the probability to detect a second photon after detection a first one is suppressed. By using two of this single photon emitters in form of trapped calcium ions, it is possible to create a ligh source consisting of two single calcium ions.
If the scattered light does not contain information of its creating ion (achieved by measuring the far-field), some interesting properties of this light source can be observed. As described in fig. 1, the two ion system can be described by the Dicke-Basis (Wolf et al. 2020)*. Interestingly the anti symmetric state |a> does not couple to the laser field. The system now shows behaviour of typical single photon emitters, an anti-bunched photon statistics, if it driven in the symmetric decay channel. If a photon is emitted in a way that the system ends up in the anti-symmetric case, it becomes invisible for the driving laser and a second photon has to be emitted to bring the system back t0 the ground state. Such behaviour of the emission of two photons in a short period of time is called bunching and can be found normally only for chaotic or thermal light sources.
Interestingly the decay channel can be chosen by the angle of investigation of the trapped ion crystal, deliviring a light source whose emission statistics can be tuned from non-classical anti-bunching to classical bunching and everything in between by just changing the angle under which it is observed.
In  the measurement was conducted by using avalanche photo diodes and a TDC (Time-To-Digital-Converter). The angle of observation was chosen by a slit. With this method the majority of the light is discarded and thus the measurement takes 2-3 days per point to gather sufficient statistics. In fig 3. the results of this measurement campaign can be seen. The acquisition of the 8 measured angles took roughly 30 days.
In  and fig.4 the LINCam was used to redo the experiment with the aim of measuring more than 8 angles. By using two synchronized LINCams it was possible to record a two-photon-event stream and correlating those events on the fly. The measurement campaign took 30 days again but delivered not only 8 observation angles, but 96. With the APD setup the campaing would have last one year.
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