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Spinning Disk Confocal Microscope | SIMTRUM Photonics

Spinning Disk Confocal Microscope
SIMSCOP SpinDisk Basic Spinning Confocal Microscope

To guarantee each laboratory greater productivity without compromising the data quality,  we have created the SpinDisk Basic, the most accessible spinning disk solution for fast  and gentle confocal imaging. The SpinDisk Basic is a truly enabling technology where the high-performance is combined with  the application flexibility and a universal compatibility with any upright and inverted  microscopes with a camera port. 


- SIMTRUM offers the  freedom to choose the  disk geometry that best  suits the application  (i.e., deep imaging, fast  live imaging).


- With the perfect match  with pinhole size and high  light throughput the SpinDisk Basic can be coupled  with LED and LASER  light source.


- Custom-designed lenses  are optimized to perform  with a wide range of  wavelengths from UV to  near NIR to use a large  variety of fluorophores.




Product Brochure Link: 



25mm Field of View

The SpinDisk Basic enables researchers to routinely perform challenging live-imaging experiments for extended periods of time. The highest spinning disk rotation on the market allows to follow ultra-fast cell dynamics with an acquisition speed of over 1000 fps on full FOV.


The multi-beam spinning method offers not only high-speed imaging but significantly reduced photo bleaching and phototoxicity. This gentle illumination combined with advanced optical sectioning makes the SpinDisk Basic the standard tool for 3D live cell imaging.


                                                                                 SpinDisk Basic Larger FOV View  


                     SpinDisk Basic Standard View 




The spinning disk allows a fluid widefield / confocal transition 

providing the true Z optical sectioning in on-click.

The wider FOV up to 25mm translates into  more information 

collected and less  tiles required to cover a large specimen.






Taking care of your samples



The SpinDisk Basic enables researchers to  routinely    

perform challenging live-imaging  experiments for   

extended periods of time.  The highest spinning disk 

rotation on the  market  allows to follow ultra-fast cell

          

The multi-beam spinning method offers not only high-speed imaging but significantly reduced photo bleaching and phototoxicity. 

This gentle illumination combined with advanced optical sectioning makes the SpinDisk Basic the standard tool for 3D live cell imaging.


SIMSCOP SpinDisk Advance Spinning Confocal Microscope

SpinDisk Advance is the next generation of spinning disk. It relies on the cutting-edge technology, advanced optical design and engineering solutions developed to meet the very high-end specifications required by modern fluorescence microscopy applications.


See Brighter –  Enhanced Sensitivity and Image Quality

The disk optimized geometry together with the carefully designed optical layout gives contrast and image clarity, ensuring brighter images of dim samples.

See Faster – High-sectioning, Hi-speed

The capacity to image a field of view up to 25mm at the maximum disk rotation, makes the SpinDisk Advance the fastest confocal microscope on the market.

See More – Dual-camera, ultra-large field of view

It is also the first confocal unit that allows dual camera imaging of the full field of view on both cameras with homogenous illumination.



Day 50 human cerebral organoid showing CTIP2 – positive deep layer cortical neurons in white and pan-neuronal MAP2 marker signal in red. Nuclei were stained with DAPI(blue).


Product Brochure Link: 






SpinDisk Advance Feature Insight
Illumination
Sealed Spinning Disk Box

Spinning Disk Synchronization


Microlenses approach makes the illumination 

field highly homogeneous on large fields of view.


A unique plug-and-play design solution allows 

you to choose and change the illumination size 

to match the most common camera formats. If 

needed it is possible to shrink the illumination on 

smaller areas.


Smaller areas mean higher power density, 

higher efficiency and higher speed.


This unique new feature makes the Spinning disk 

fully isolated and protected from environmental 

dust and prevents undesired artifacts related to 

the presence of small dust particles on the 

Spinning disk surface.


To manage very high-speed imaging, hundreds 

of fps, SpinDisk Advance provides as a standard 

feature a synchronization signal from the spinning 

disk to the camera.


A good way to make a solid quantitative analysis 

of very fast dynamics.


Multiple Modalities

The SpinDisk Advance is designed to be a truly enabling technology where performance is combined with the flexibility of a modular, expandable system. To achieve this, the SpinDisk Advance has three modes of use:


The motorized IN/OUT disk

allows for a fluid

widefield/confocal

transition without the

need for realignment.


WIDEFIELD

With the SpinDisk Advance 

every upright or inverted

microscope can benefit from

confocal’s advanced optical

sectioning capacity.


SPINNING DISK

CONFOCAL

The system is designed to 

be compatible with the 

SpinDisk SIM add-on, 

for a seamless evolution 

from confocal to super-resolution.


SUPER-RESOLUTION






Largest FOV in the market
          

New scientific discoveries often depend on  imaging and

analyzing large populations of  cells to obtain robust data.


With the largest field-of-view (FOV) for  both inverted and 

upright microscopes (25  mm   diagonal), more specimens 

fit in one  FOV with more objective lens choices than ever before.


Biology in 3D involves more than monolayers  of cells;

the SpinDisk Advance is designed to capture  spheroids 

and full organisms in a single FOV.

Lung cells, DAPI for DNA (blue), laminin in green,          

F-actin in red, CFI  Plan Apo Lambda 60X oil, 1.4 NA.       




                   

Retinal ganglion cells, DAPI for DNA (white) and            

three differentiation  markers, MAP2 (yellow),               

Tuj1 (magenta), Brn3a (cyan). 40x water  objective,          

 NA 1.25                                            

           Nematodes expressing a pan-neuronal genetically 

                      encoded fluorescent Ca2+ indicator, 

                              25X SIL 1.05 NA objective.



Brain organoid, stitched image: unclear transcription factor TBR1 (Alexa 750

fluorophore, shown in magenta), microtubule-associated protein MAP2 (Alexa 488

fluorophore, shown in green), DAPI for DNA in blue. Plan Apochromat Lambda 60X oil.


The wider FOV allows the imaging of a sample area almost double the size of conventional imaging  systems. A wider area translates into more information collected from each image and a reduction  in the number of tiles needed to cover a large sample, significantly accelerating the research  process. Further acceleration is provided by the dual-camera functionality.



Uniform illumination for data quantification

Homogeneous illumination over the entire field of view is essential for quantitative imaging. The SpinDisk Advance illuminator is based on micro-lens technology which is able to turn a high-power laser  from a multi-mode fiber into a uniform square collimated beam with over 90% homogenous  illumination over the entire 25mm FOV. This unique feature allows data quality and data  throughput to be increased avoiding artifacts and recovering information even from the  periphery.


                                                                                                       Cos-7 cells with mitochondria in green

                                                                                                                                          (Anti-Tom20 with OG 488). (A)

                                                                                                                                          Illumination without micro-lenses.(B)

                                                                                                                                          FOV illumination with micro-lenses,

                                                                                                                                          Objective 60X oil, 1.4 NA

  Power distribution over the FOV

  with micro-lenses illuminator




Seamless stitching of large samples
Combined with the 25mm FOV, micro lenses are essential for seamless stitching of  images of large samples like tissues, organoids and full organisms. This ability allows you  to:

● get reliable data without artifacts, avoiding any post-processing correction

● reduce photobleaching and increase speed by minimizing the overlap of tiles

● increase data throughput and guarantee data quality

Traditional

illuminator

SpinDisk Advance 

Micro-lenses  

illuminator

Seamless stitching of mouse kidney tissue section with Alexa 488 WGA, Alexa 568 Phalloidin



Tailor made solutions

The spinning disk-box is one of the most versatile components of the SpinDisk Advance. SIMTRUM  offers the freedom to choose the disk geometry that best suits your applications,  including complete disk customization. The 50 µm diameter spinning disk is available in three  different versions:


    

High Throughput (HT) Slits

geometry is designed for 

samples that require high

speed and low excitation 

power.



    

The standard disk is the ideal 

balance between brightness and 

confocality for a wide range of 

applications during routine 

research.



    

The wider spacing version has 

been designed to further 

enhance the image quality and 

sectioning in thicker specimens 

thanks to the large reduction of 

crosstalk between pinholes.

A. *see zoom description on the following page B. MIP from Z stack of a cancer spheroid, living cells are marked with Calcein (green), 

dead cells  with PI (red), nuclei are stained with Hoechst (blue). 25X SIL 1.05 NA objective. C. Clarified mammary gland tissue, 

3D volume view 500 µm thick,  showing keratine 8 (red) keratine 5 (yellow) and DAPI (blue). 25X SIL 1.05 NA objective.


Dual color, fast imaging, still wider

A crucial issue in imaging live samples is the ability to localize small multicolor fluorescent  signals and track them while moving at high speed. With the highest spinning disk rotation  on the market (15K rpm), the SpinDisk Advance offers an achievable speed of over 1000fps on full FOV, over 10-time faster acquisition compared to the fastest point scanning confocal.

Thanks to the default dual-camera conguration, the SpinDisk Advance allows you to run simultaneous multi-color imaging on a FOV of 25mm on both cameras.



Product specifications and Brochures



ParametersSpinDisk Basic
SpinDisk Advance
SpinDisk SIM
Imaging ModalitiesWidefield / ConfocalWidefield / Confocal / Super-resolution
(in combination with SIM Basic add-on module)
Super-resolution | Confocal spinning disk | Widefield
Compatible MicroscopesFull range of upright and   inverted microscopesFull range of upright and   inverted microscopesInverted microscopes
FOVUp to 25mm diameterUp to 25mm diameter1024 x 1024pixel (66 x 66 µm   100X | 333 x 333µm 20X)
Light SourceLASER with 1,5mm SMA | LED with   3mm LLGMulti mode lasers with SMA   couplingMulti mode lasers with SMA   coupling
Spectral Range

Excitation 400-750 nm / Emission

 400-850 nm / Excitation 400-1000nm

Excitation 400-750 nm / Emission   400-850 nmExcitation: 400-750 nm;   emission: 400-850 nm
Camera

Compatible with CCD/EMCCD cameras

 and sCMOS cameras

Compatible with CCD/EMCCD  cameras and sCMOS cameras 

Dual-camera view: simultaneous dual-camera imaging up to 25 mm

Any triggerable camera having   6.5 µm pixel size
Dual camera option available*
Disk Speed / Scan Rate15000 RPM / > 1000 fps15000 RPM / > 1000 fps13fps (1024 x 1024 px)
Spinning Disk Geometry 
(Diamater/Spacing)
50/250 slit for high throughput   & live imaging applications 
50/250 µm pinholes optimized for LASER light source 
60/220 µm pinholes optimized for LED light source
Custom geometry and double pattern disks available on demand
50/250 slit spirals for high   throughput applications 
50/250 µm pinholes for routine imaging
50/400 µm with wider spacing for deep imaging
50/250 slit spirals for high   throughput applications 
50/250 µm pinholes for routine imaging
50/400 µm with wider spacing for deep imaging
ResolutionLateral Resolution (FWHM): ~230   nm (High NA 1.4) 
Axial Resolution (FWHM): ~600 nm (High NA 1.4)
Lateral Resolution (FWHM): ~230   nm (High NA 1.4) 
Axial Resolution (FWHM): ~600 nm (High NA 1.4)
Lateral Resolution (FWHM): ~100   nm (100X NA 1.45)
Axial Resolution (FWHM): ~300 nm (100X NA 1.45)
Filter WheelsMotorized filter wheels:   4-positions cleanup filter wheel, 3-positions    dichroic filter wheel, 8-positions emission filter wheel.Motorized filter wheels and   slider: 4-positions cleanup filter wheel, 3-positions dichroic filter wheel, 8-positions emission filter wheel, 3 positions slider for dual   camera useMotorized filter wheels and slider: 4-positions cleanup filter wheel,  3-positions dichroic filter wheel, 8-positions emission filter wheel,  3 positions slider for dual camera use
SoftwareNikon NIS-Elements / Micro-Manager / MetaMorph / Volocity / VisiViewNikon NIS-Elements / Micro-Manager / MetaMorph / Volocity / VisiViewµManager /VisiView® / NIS Elements
Recommended   Installation ConditionsTemperature 23 ± 5˚C, Humidity 70% RH or less (no condensation)Temperature 23 ± 5˚C, Humidity 70% RH or less (no condensation)Temperature 23 ± 5°C, Humidity 70% RH or less
Weight11.0 Kg (24.3 lbs)26.0 Kg (57 lbs)20 Kg (44 Ibs)
Dimensions11.2 (w) x 15.9 (L) x 8.9 (h)   inches
 286.7 (w) x 405.1 (L) x 226.5 (h) mm
14 (w) x 23.9 (L) x 8.9 (h)   inches
357.0 (w) x 606.0 (L) x 225.0 (h) mm
14.0 (w) x 17.1 (L) x 11.4 (h)   inches
356.0 (w) x 435.0 (L) x 290.5 (h) mm
Layout



Parameters

Basic

P Series Box

Basic

P Series

Advance

P Series

Basic

L Series

Advance

L Series

SpinDisk

Basic

SpinDisk

 Advance

SIM BasicSpinDisk SIM
Image Frame Rate4fps@512x5124fps@512x5124fps@512x51210fps@1024x102414fps@1024x1024100fps@2048x2048100fps@2048x204813fps@1024x102413fps@1024x1024
Resolution~230 nm ~230 nm ~230 nm ~230 nm 150-200nm~230 nm ~230 nm ~100 nm ~100 nm 
Image Depth<100µm<100µm<100µm<100µm<600µm<200µm<200µm<50µm<200µm
No. of Laser 114144444
Wavelength Choice 

405/445/488

/525/561/640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

DetectorsDetectorDetectorDetectorCMOS CamerasCMOS CamerasCMOS CameraDual sCMOS Camera(Single sCMOS optional)sCMOS CameraDual sCMOS Camera(Single sCMOS optional)
Microscope NonInverted (Upright Customizeable)Inverted (Upright Customizeable)Inverted (Upright Customizeable)Inverted (Upright Customizeable)

Inverted or

Upright 

Inverted or

Upright 

Inverted or

Upright 

Inverted
StagesNon

Z Motorized

XY Manual

XYZ Motorized Z Motorized
XY Manual
XYZ Motorized XYZ Motorized XYZ Motorized XYZ Motorized XYZ Motorized 
Electronics system

Motorized Filter
Motorized Pin hole

Motorized FilterMotorized FilterMotorized FilterMotorized FilterMotorized Filter
image contrastMediumMediumHighMediumHighHighHighHighHigh
Upgrade optionNonNonUpgrade to 30fps high Speed NonNonNonUpgrade to SPIN Disk SIM 100nm resolutionUpgrade to SPIN Disk SIM 100nm resolutionNon
Customized Option

Yes
YesYesYes

ApplicationsBiomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning;Biomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning;
Biomedicine: three-dimensional fluorescence microscopy imaging observation of cells and tissues, multi-channel fluorescence detection
Reflective 3D microscopic imaging, surface shape detection,   rapid analysis and measurement of materials and microelectronic device   surface shapeFast 3D imaging of thick tissue, 3D histopathological image   detection, 3D histomorphological research of small animal tissues and organs   such as brain neurons, liver, kidney, etc.Dynamic fluorescence imaging of living cells/tissues,   microscopic observation of small animals such as zebrafish and nematodesDynamic fluorescence imaging of living cells/tissues,   microscopic in vivo observation of small animals such as zebrafish and   nematodes, and in vivo observation of multi-channel fluorescent organelles.Dynamic fluorescence super-resolution imaging of living cells   can be used for live cell dynamics, allowing tracking of biological changes   at cellular and subcellular levelsDynamic fluorescence super-resolution imaging of living cells,   3D imaging of tissues, organoids, spheroids and small organisms.



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Compare Model Drawings & Specs Availability Reference Price
(USD)
SpinDisk Basic
Spinning Disk Confocal Microscope, SpinDisk Basic Series, Image frame rate 100fps@2048x2048, Resolution ~230nm, image depth <200um, No. of Laser - 4, Wavelength choice 405/445/488/525/561/640nm, sCMOS Camera detectors, Inverted or upright microscope, XYZ motorized stages, high image contrast, customized option
6-10 Weeks Request for quote
SpinDisk Advance
Spinning Disk Confocal Microscope, SpinDisk Advance Series, Image frame rate 100fps@2048x2048, Resolution ~230nm, image depth <200um, No. of Laser - 4, Wavelength choice 405/445/470/520/528/555/640nm, Dual sCMOS Camera detectors (single sCOMS optional), Inverted or upright microscope, XYZ motorized stages, Motorized filter electronics system, high image contrast, upgrade to SpinDisk SIM 100nm resolution, customized option
6-10 Weeks Request for quote

SpinDisk Advance - Parameter

SpinDisk Basic - Parameter

SpinDisk Advance - Download

SpinDisk Basic - Download

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