Spinning Disk Confocal Microscope

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Products / Microscopes /Specialized Microscopes /Spinning Disk Confocal Microscope /

Spinning Disk Confocal Microscope

SpinDisk Basic SpinDisk Advance Specifications Applications Related Products Order Now!

SIMSCOP SpinDisk Basic Spinning Confocal Microscope

To guarantee each laboratory greater productivity without compromising the data quality, we have created the SpinDisk Basic, the most accessible spinning disk solution for fast and gentle confocal imaging.

The SpinDisk Basic combines high performance and flexibility. Offering customers the freedom to choose the turntable geometry that best suits their application (depth imaging, fast real-time imaging).

Compatible with any upright or inverted microscope with a camera port.

With the perfect match with pinhole size and high light throughput the SpinDisk Basic can be coupled with LED and LASER light source.

Custom-designed lenses are optimized to perform with a wide range of wavelengths from UV to near NIR to use a large variety of fluorophores.

Product Brochure Link:

SpinDisk Basic Light Path Diagram

The wider FOV up to 25mm translates into more information

collected and less tilles required to cover a large specimen.

The SpinDisk Basic enables researchers to routinely perform challenging live-imaging experiments for extended periods of time. The highest spinning disk rotation on the market allows to follow ultra-fast cell.		The multi-beam spinning method offers not only high-speed imaging but significantly reduced photo bleaching and phototoxicity. This gentle illumination combined with advanced optical sectioning makes the SpinDisk Basic the standard tool for 3D live cell imaging.

Furthermore, our XY Microscope Piezo Stages has been designed for use our SIMSCOP Series Microscopes to provide motorized XY positioning of microscopy samples. Access for SIMTRUM's XY Microscope Stages can be click here.

SIMSCOP SpinDisk Advance Spinning Confocal Microscope

SpinDisk Advance is the next generation of spinning disk. It relies on the cutting-edge technology, advanced optical design and engineering solutions developed to meet the very high-end specifications required by modern fluorescence microscopy applications.

See Brighter – Enhanced Sensitivity and Image Quality

The disk optimized geometry together with the carefully designed optical layout gives contrast and image clarity, ensuring brighter images of dim samples.

See Faster – High-sectioning, Hi-speed

The capacity to image a field of view up to 25mm at the maximum disk rotation, makes the SpinDisk Advance the fastest confocal microscope on the market.

See More – Dual-camera, ultra-large field of view

It is also the first confocal unit that allows dual camera imaging of the full field of view on both cameras with homogenous illumination.

SpinDisk Advance Light Path Diagram

SIMSCOP_SpinDisk_A_Optical_Layout

SIMSCOP_SpinDisk_A010

Day 50 human cerebral organoid showing CTIP2 - positive deep layer cortical neurons in white and pan-neuronal MAP2 marker signal in red. Nuclei were stained with DAPI(blue).

Multiple Modalities

The SpinDisk Advance is designed to be a truly enabling technology where performance is combined with the flexibility of a modular, expandable system. To achieve this, the SpinDisk Advance has three modes of use:

Left: Widefield - the motorized IN/OUT disk allows for a fluid widerfield/confocal transition without the need for realignment.

Middle: Spinning Disk Confocal -with SpinDisk Advance every upright or inverted microscope can benefit from confocal's advanced optical sectioning capacity.

Right: Super-resolution - the system is designed to be compatible with the SIM SpinDisk add-on, for a seamless evolution from confocal to super-resolution.

Largest FOV in the market

Due to the extensive field of view (FOV) encompassed by the SpinDisk Advance, the sample area is nearly twice as expansive as that of traditional imaging systems. This broader area translates into the acquisition of a higher quantity of data from each image, thereby reducing the requisite amount of sheet material for covering extensive samples. This significantly accelerates the research process. Additionally, the capacity for dual camera utilization serves to further expedite the pace of research.

Left: Brain organoid, stitched image: Unclear transcription factor TBR1 (Alexa 750 fluorophore, shown in magenta), microtubule-associated protein MAP2 (Alexa 488 fluorophore, shown in green), DAPI for DNA in blue. Plan Apochromat Lambda 60X oil.

Uniform illumination for data quantivication

Homogeneous illumination over the entire field of view is essential for quantitative imaging. The SpinDisk Advance illuminator is based on micro-lens technology which is able to turn a high-power laser from a multi-mode fiber into a uniform square collimated beam with over 90% homogenous illumination over the entire 25mm FOV. This unique feature allows data quality and data throughput to be increased avoiding artifacts and recovering information even from the periphery.

Seamless stitching of mouse kidney tissue section with Alexa 488 WGA, Alexa 568 Phalloidin

Seamless stitching of large samples

Combined with the 25mm FOV, micro lenses are essential for seamless stitching of images of large samples like tissues, organoids and full organisms. This ability allows you to:

Get reliable data without artifacts, avoiding any post-processing correction
Reduce photobleaching and increase speed by minimizing the overlap of tiles
Increase data throughput and guarantee data quality

Product specifications and Brochures

Product Brochure Link:

Parameters	SpinDisk Series - Basic	SpinDisk Series - Advance
Imaging Modalities	Widefield / Confocal / Fluorescence	Widefield / Confocal / Super-resolution (in combination with SIM Basic)
Compatible Microscopes	Full range of upright and inverted microscopes	Full range of upright and inverted microscopes
FOV	Up to 25mm diameter Plan apochromatic objectives 4x - 2.7mm x 2.7mm Plan apochromatic objectives 10x – 1.20mm x 1.20mm Plan apochromatic objectives 20x - 0.67mm x 0.67mm Plan apochromatic objectives 40x - 0.33mm x 0.33mm Plan apochromatic objectives 60x - 220µm x 220µm Plan apochromatic objectives 100x - 130µm x 130µm
Laser	Multimode laser: 1.5mm SMA \| LED: 3mm LLG LED light source life >20,000 hours Power adjustment accuracy 1%	Multi model lasers with SMA coupling
Spectral Range	Excitation 365 -750 nm / Emission 400 - 850 nm / Excitation 400 - 1000nm	Excitation 400-750 nm / Emission 400-850 nm
Camera	sCMOS Camera, compatible with CCD/EMCCD camera	sCMOS camera, compatible with CCD/EMCCD camera Support dual-channel dual-camera imaging
Camera Index	Peak quantum efficiency 95%@600nm； Pixel size 6.5µm x 6.5µm；Resolution 2048x2048；Effective Area 13.3x13.3mm； Noise: 0.2e-；Full well capacitance: 45ke-; Compatible with aire cooling/water cooling
Disk Speed	15000 RPM	15000 RPM
Scan Rate	100 - 1000 fps	100 - 1000 fps
Spinning Disk Geometry (Diameter / Spacing)	50/250 slit for high throughput & live imaging applications 50/250 µm pinholes optimized for Laser light source 60/220 µm pinholes optimized for LED light source Customized geometry and dual pattern disks available on demand	50/250 slit spirals for high throughput applications 50/250 µm pinholes for routine imaging 50/400 µm with wider spacing for deep imaging
Resolution	Lateral Resolution (FWHM): ~230 nm (High NA 1.4) Axial Resolution (FWHM): ~600 nm (High NA 1.4)	Lateral Resolution (FWHM): ~230 nm (High NA 1.4) Axial Resolution (FWHM)：~600 nm (High NA 1.4)
Filter Wheels	8-position excitation filter wheel; 5-position dichroic filter wheel; 8-position emission filter wheel; Spindisk/Wide-field motorized switching	4-positions sleanup filter wheel, 3-positions dichroic filter wheel, 8-positions emission filter wheel, 3-position slider for dual camera
Filter Unit	DAPI EM 445nm/50nm \| GFP/ FITC EM 530nm/50nm DsRed / TRITC EM 605nm/60nm \| Cy5 EM 695nm/40nm
Eyepiece	WF 10X/23 plan eyepiece, high eye point; centering telescope, 45°inclined, interpupillary distance adjustment 50‒75mm, adjustable diopter
Objective Converter	Converter with five-hole internal positioning; Ball bearing for internal position ning
Stage	Manual: 240mm× 260mm fixed stage; Range of movement: 135mm×85mm Motorized: Minimum step size: 50nm; Stroke: X:114mm Y:75mm；Repeatability：±0.1um; Maximum speed: ≥100mm/s；Stage size: ≥270x170mm Maximum load capacity＞1KG (Horizontal)
Z Driver	Focusing resolution minimum step size: 0.05μm; Repeatability ±0.2μm; Maximum stroke: 10mm
Focusing Mechanism	Coaxial coarse/ fine adjustment with limit and locking devices, Low level coaxial focusing handwheel ; Handwheel graduations of fine adjustment:1μm
Software Feature	Multi-color fluorescence localization processing；Z-stack data processing; Large image stitching； Image analysis; Imaging data management；3D imaging constrution
Recommended Installation Conditions	Temperature 23 ± 5˚C, Humidity 70% RH or less (no condensation)	Temperature 23 ± 5˚C, Humidity 70% RH or less (no condensation)
Weight	11.0 Kg (24.3 lbs)	26.0 Kg (57 lbs)
Size	11.2 (W) x 15.9 (L) x 8.9 (H) inches \| 286.7 (W) x 405.1 (L) x 226.5 (H) mm	14 (W) x 23.9 (L) x 8.9 (H) inches \| 357.0 (W) x 606.0 (L) x 225.0 (H) mm

SpinDisk Confocal Microscopy Acquistion Atlas

High-Definition Visual Assets: Should you require high-definition authentic images and video footage, we invite you to get in touch with us.

Mouse nerve 3D tomography, 20X objective lens, scanning depth ~100 microns

(Original Image)

Mouse nerve tomography 3D reconstruction, 20X objective lens, scanning depth ~100 microns

Mouse nerve 3D tomographic scanning, 60X objective lens, scanning depth ~100 microns

(Original Image)

Mouse nerve tomography 3D reconstruction, 60X objective lens, scanning depth ~100 microns

(The picture has been processed with background noise reduction)

Zebrafish 3D tomography (Microscope system uses Olympus IX83)

Cell mitosis CETN1-GFP green, SiR-DNA blue (Microscope system uses Nikon Ti2)

BPAE cells with MitoTracker^TM Red CMXRos, Alexa Fluor^TM 488 Phalloidin, and DAPI

BPAE cells, DAPI, 60X ojective Lens (Original Image)

BPAE cells with MitoTracker^TM Red CMXRos, Alexa Fluor^TM 488 Phalloidin, and DAPI

BPAE cells, FITC, 60X ojective Lens (Original Image)

BPAE cells with MitoTracker^TM Red CMXRos, Alexa Fluor^TM 488 Phalloidin, and DAPI

BPAE cells, CY5, 60X ojective Lens (Original Image)

BPAE cells with MitoTracker^TM Red CMXRos, Alexa Fluor^TM 488 Phalloidin, and DAPI

BPAE cells, DAPI + FITC + CY5, 60X ojective Lens (Original Image)

BPAE cells with Mouse Anti-α-tubulin, Alexa Fluor^TM 488 Phalloidin, and DAPI

BPAE cells, DAPI, 60X ojective Lens (Original Image)

BPAE cells with Mouse Anti-α-tubulin, Alexa Fluor^TM 488 Phalloidin, and DAPI

BPAE cells, FITC, 60X ojective Lens (Original Image)

BPAE cells with Mouse Anti-α-tubulin, Alexa Fluor^TM 488 Phalloidin, and DAPI

BPAE cells, CY5, 60X ojective Lens (Original Image)

BPAE cells with Mouse Anti-α-tubulin, Alexa Fluor^TM 488 Phalloidin, and DAPI

BPAE cells, DAPI + FITC + CY5, 60X ojective Lens (Original Image)

BPAE cells1-100X-SpinDisk-405&488&525

BPAE cell 1, 100X objective lens, Laser 405,488 & 525

(Original Image)

BPAE cells2-100X-SpinDisk-405&488&525

BPAE cell 2, 100X objective lens, Laser 405,488 & 525

(Original Image)

Cyto-Golgi scan, 100X objective lens

(Original Image)

Cyto-Golgi scan, 100X objective lens

(Original Image)

Hippocampal_coronal_slice, 60micron thickness

Mouse neural tomography, 10X objective lens, wide field effect

(Original Image)

Mouse nerve tomography, 10X objective lens, turntable effect

(Original Image)

Mouse nerve tomography, 40X objective lens, wide field effect

(Original Image)

Mouse nerve tomography, 40X objective lens, turntable effect

(Original Image)

Mouse nerve tomography, 60X objective lens, multi-image superimposed effect in Z direction, superimposed depth ~100 microns

Mouse neural tomography, 100X objective lens

( Original Image)

Spinach rhizome, 20X objective lens

(Original Image)

Plant leaf stems, 20X objective lens

(Original Image)

Sago palm leaf, 20X objective lens

(Original Image)

Wheat seeds, 20X objective lens

(Original Image)

Parameters	Basic P Series Box	Basic P Series	Advance P Series	Basic L Series	Advance L Series	SpinDisk Basic	SpinDisk Advance	SIM Basic	SpinDisk SIM
Image Frame Rate	4fps@512x512	4fps@512x512	4fps@512x512	10fps@1024x1024	14fps@1024x1024	100fps@2048x2048	100fps@2048x2048	13fps@1024x1024	13fps@1024x1024
Resolution	~230 nm	~230 nm	~230 nm	~230 nm	150-200nm	~230 nm	~230 nm	~100 nm	~100 nm
Image Depth	<100µm	<100µm	<100µm	<100µm	<600µm	<200µm	<200µm	<50µm	<200µm
No. of Laser	1	1	4	1	4	4	4	4	4
Wavelength Choice	405/445/488 /525/561/640(nm)	405/445/488/ 525/561/640(nm)	405/488/561/ 640(nm)	405/445/488/ 525/561/640(nm)	405/488/561/ 640(nm)	405/445/470/520/ 528/555/640(nm)	405/445/470/520/ 528/555/640(nm)	405/445/470/520/ 528/555/640(nm)	405/445/470/520/ 528/555/640(nm)
Detectors	Detector	Detector	Detector	CMOS Camera	sCMOS Camera	sCMOS Camera	Dual sCMOS Camera（Single sCMOS optional）	sCMOS Camera	Dual sCMOS Camera（Single sCMOS optional）
Microscope	Non	Inverted (Upright Customizeable)	Inverted (Upright Customizeable)	Inverted (Upright Customizeable)	Inverted (Upright Customizeable)	Inverted or Upright	Inverted or Upright	Inverted or Upright	Inverted
Stages	Non	Z Motorized XY Manual	XYZ Motorized	Z Motorized XY Manual	XYZ Motorized	XYZ Motorized	XYZ Motorized	XYZ Motorized	XYZ Motorized
Electronics system			Motorized Filter Motorized Pin hole		Motorized Filter	Motorized Filter	Motorized Filter	Motorized Filter	Motorized Filter
image contrast	Medium	Medium	High	Medium	High	High	High	High	High
Upgrade option	Non	Non	Upgrade to 30fps high Speed	Non	Non	Non	Upgrade to SPIN Disk SIM 100nm resolution	Upgrade to SPIN Disk SIM 100nm resolution	Non
Customized Option			Yes		Yes	Yes	Yes
Applications	Biomedicine: monochromatic fluorescence microscopic observation of cells, 3D scanning;	Biomedicine: monochromatic fluorescence microscopic observation of cells, 3D scanning;	Biomedicine: three-dimensional fluorescence microscopy imaging observation of cells and tissues, multi-channel fluorescence detection	Reflective 3D microscopic imaging, surface shape detection, rapid analysis and measurement of materials and microelectronic device surface shape	Fast 3D imaging of thick tissue, 3D histopathological image detection, 3D histomorphological research of small animal tissues and organs such as brain neurons, liver, kidney, etc.	Dynamic fluorescence imaging of living cells/tissues, microscopic observation of small animals such as zebrafish and nematodes	Dynamic fluorescence imaging of living cells/tissues, microscopic in vivo observation of small animals such as zebrafish and nematodes, and in vivo observation of multi-channel fluorescent organelles.	Dynamic fluorescence super-resolution imaging of living cells can be used for live cell dynamics, allowing tracking of biological changes at cellular and subcellular levels	Dynamic fluorescence super-resolution imaging of living cells, 3D imaging of tissues, organoids, spheroids and small organisms.


Wide Field Raman Microscope	Fluorescence / Photoluminescence Microscope	Time Correlated Single Photon Counting (TCSPC) For SPAD Testing

XY Microscope Piezo Stages
Model	ST-XY3-11075	ST-XY2-7550
Image
Stroke(mm)	110 X 75	75 X 50
Driver	Piezoelectric Motor	Piezoelectric Motor
Maximum Load Capacity(KG)	1	1
Maximum Speed(mm/s)	100	100
Minimum Step Size(nm)	20 (50, 100nm for option)	50
Repeatability(nm)	+/-200	+/-200
Position Sensor	Grating	Grating
Material	Aluminum Alloy	Aluminum Alloy
Weight(KG)	3.3	2.5

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Compare	Model		Drawings & Specs	Availability	Reference Price (USD)
		SpinDisk Basic Spinning Disk Confocal Microscope Spinning Disk Confocal Microscope, SpinDisk Basic Series, Image frame rate 100fps@2048x2048, Resolution ~230nm, image depth <200um, No. of Laser - 4, Wavelength choice 405/445/488/525/561/640nm, sCMOS Camera detectors, Inverted or upright microscope, XYZ motorized stages, high image contrast, customized option		6-10 Weeks	Request for quote
		SpinDisk Advance Spinning Disk Confocal Microscope, SpinDisk Advance Series, Image frame rate 100fps@2048x2048, Resolution ~230nm, image depth <200um, No. of Laser - 4, Wavelength choice 405/445/470/520/528/555/640nm, Dual sCMOS Camera detectors (single sCOMS optional), Inverted or upright microscope, XYZ motorized stages, Motorized filter electronics system, high image contrast, upgrade to SpinDisk SIM 100nm resolution, customized option		6-10 Weeks	Request for quote