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Spinning Disk Confocal Microscope | SIMTRUM Photonics

Spinning Disk Confocal Microscope
SIMSCOP SpinDisk Basic Spinning Confocal Microscope

 

To guarantee each laboratory greater productivity without compromising the data quality,  we have created the SpinDisk Basic, the most accessible spinning disk solution for fast  and gentle confocal imaging.

 

The SpinDisk Basic combines high performance and flexibility. Offering customers the freedom to choose the turntable geometry that best suits their application (depth imaging, fast real-time imaging).

 

 

  • Compatible with any upright or inverted microscope with a camera port.

 

  • With the perfect match  with pinhole size and high  light throughput the SpinDisk Basic can be coupled  with LED and LASER  light source.

 

  • Custom-designed lenses  are optimized to perform  with a wide range of  wavelengths from UV to  near NIR to use a large  variety of fluorophores.

 

 

 

 

Product Brochure Link: 

 

 

  SpinDisk Basic Light Path Diagram
FOV up to 25mm SpinDisk Basic Light Path Diagram

 

The wider FOV up to 25mm translates into more information

collected and less tilles required to cover a large specimen.

 
 
The SpinDisk Basic enables researchers to routinely perform challenging live-imaging experiments for extended periods of time. The highest spinning disk rotation on the market allows to follow ultra-fast cell.   The multi-beam spinning method offers not only high-speed imaging but significantly reduced photo bleaching and phototoxicity. This gentle illumination combined with advanced optical sectioning makes the SpinDisk Basic the standard tool for 3D live cell imaging.
SIMSCOP_SpinDisk_001   SIMSCOP_SpinDisk_002

Furthermore, our XY Microscope Piezo Stages has been designed for use our SIMSCOP Series Microscopes to provide motorized XY positioning of microscopy  samples. Access for SIMTRUM's XY Microscope Stages can be click here.


SIMSCOP SpinDisk Advance Spinning Confocal Microscope

SpinDisk Advance is the next generation of spinning disk. It relies on the cutting-edge technology, advanced optical design and engineering solutions developed to meet the very high-end specifications required by modern fluorescence microscopy applications.

 

  • Brighter - intense flexibility and the quality of pictures The spinning disk's geometry is optimized while being endowed with a carefully designed optical layout structure, which showcases contrast and image sharpness, ensuring that the image brightness of dim samples is brighter.
  • Faster - High speed slice With a spinning disk capable of imaging a field of view up to 25mm wide at maximum rotation speed, the SpinDisk Advance is the fastest confocal microscope on the market.
  • More - two cameras huge FOV SpinDisk Advance is the first dual camera imaging confocal unit, in the case of uniform illumination, the entire field of view can be observed in both cameras.

 

SpinDisk Advance Light Path Diagram 

SIMSCOP_SpinDisk_A_Optical_Layout

 

 

SIMSCOP_SpinDisk_A010

3D biology is much more than a single layer of cells,SpinDisk  Advance is designed to capture spheroids and whole organisms in a single field of view. New scientific discoveries often rely on imaging and analyzing large numbers of cells to obtain reliable data. SpinDisk Advance offers the largest field of view (25mm diagonal) in both upright and inverted microscopes, is able to accommodate more specimens in a single field of view, and offers more objective options than ever before.

Multiple Modalities

 

The SpinDisk Advance is designed to be a truly enabling technology where performance is combined with the flexibility of a modular, expandable system. To achieve this, the SpinDisk Advance has three modes of use:

SIMSCOP_SpinDisk_A_MM01

Left: Widefield - the motorized IN/OUT disk allows for a fluid widerfield/confocal transition without the need for realignment.

Middle: Spinning Disk Confocal -with SpinDisk Advance every upright or inverted microscope can benefit from confocal's advanced optical sectioning capacity.

Right: Super-resolution - the system is designed to be compatible with the SIM SpinDisk add-on, for a seamless evolution from confocal to super-resolution.

 

SIMSCOP_SpinDisk_A_FOV

Largest FOV in the market

Due to the extensive field of view (FOV) encompassed by the SpinDisk Advance, the sample area is nearly twice as expansive as that of traditional imaging systems. This broader area translates into the acquisition of a higher quantity of data from each image, thereby reducing the requisite amount of sheet material for covering extensive samples. This significantly accelerates the research process. Additionally, the capacity for dual camera utilization serves to further expedite the pace of research.

 

Left: Brain organoid, stitched image: Unclear transcription factor TBR1 (Alexa 750 fluorophore, shown in magenta), microtubule-associated protein MAP2 (Alexa 488 fluorophore, shown in green), DAPI for DNA in blue. Plan Apochromat Lambda 60X oil.

Uniform illumination for data quantivication

 

Uniform illumination of the overall field of view is critical for quantitative imaging, and SpinDisk Advance's illuminator is able to convert high-power lasers in multimode fibers into a uniform square collimated beam, resulting in uniform illumination over 90% of the 25mm wide field of view. This unique feature improves data quality and data throughput while avoiding artifacts and even recovering information from the edge of the field of view.

SIMSCOP_SpinDisk_A_UIDQ

SIMSCOP_SpinDisk_A_LS

Seamless stitching of mouse kidney tissue section with Alexa 488 WGA, Alexa 568 Phalloidin

Seamless stitching of large samples

 

Combined with the 25mm FOV, micro lenses are essential for seamless stitching of  images of large samples like tissues, organoids and full organisms. This ability allows you  to:

  • Get reliable data without artifacts, avoiding any post-processing correction
  • Reduce photobleaching and increase speed by minimizing the overlap of tiles
  • Increase data throughput and guarantee data quality

 

 

Furthermore, our XY Microscope Piezo Stages has been designed for use our SIMSCOP Series Microscopes to provide motorized XY positioning of microscopy  samples. Access for SIMTRUM's XY Microscope Stages can be click here.


Product specifications and Brochures

Product Brochure Link: 

 

Parameters SpinDisk Series - Basic SpinDisk Series - Advance
Imaging Modalities Widefield / Confocal / Fluorescence Widefield / Confocal / Super-resolution
(in combination with SIM Basic)
Compatible Microscopes Full range of upright and inverted microscopes Full range of upright and  inverted microscopes
FOV Up to 25mm diameter
Plan apochromatic objectives 4x - 2.7mm x 2.7mm  
Plan apochromatic objectives  10x – 1.20mm x 1.20mm
Plan apochromatic objectives  20x - 0.67mm x 0.67mm
Plan apochromatic objectives 40x - 0.33mm x 0.33mm  
Plan apochromatic objectives  60x - 220µm x 220µm   
Plan apochromatic objectives 100x - 130µm x 130µm
Laser Multimode laser: 1.5mm SMA  |  LED: 3mm LLG
LED light source life >20,000 hours
Power adjustment accuracy 1%
Multi model lasers with SMA coupling
Spectral Range Excitation 400-750 nm / Emission 400-850 nm
Camera sCMOS Camera, compatible with CCD/EMCCD camera sCMOS camera, compatible with CCD/EMCCD camera
Support dual-channel dual-camera imaging
Camera Index Peak quantum efficiency 95%@600nm;
Pixel size 6.5µm x 6.5µm;Resolution 2048x2048;Effective Area 13.3x13.3mm;
Noise: 0.2e-;Full well capacitance: 45ke-;
Compatible with aire cooling/water cooling
Disk Speed 15000 RPM 15000 RPM
Scan Rate 100 - 1000 fps 100 - 1000 fps
Spinning Disk Geometry
(Diameter / Spacing)
50/250 slit for high throughput & live imaging applications
50/250 µm pinholes optimized for Laser light source
60/220 µm pinholes optimized for LED light source
Customized geometry and dual  pattern disks available on demand
50/250 slit spirals for high throughput applications 
50/250 µm pinholes for routine imaging
50/400 µm with wider spacing for deep imaging
Resolution Lateral Resolution (FWHM): ~230 nm (High NA 1.4)
Axial Resolution (FWHM): ~600 nm (High NA 1.4)
Lateral Resolution (FWHM): ~230 nm (High NA 1.4)
Axial Resolution (FWHM):~600 nm (High NA 1.4)
Filter Wheels 8-position excitation filter wheel; 5-position dichroic filter wheel;
8-position emission filter wheel; Spindisk/Wide-field motorized switching
4-positions sleanup filter wheel, 3-positions dichroic filter wheel, 8-positions emission filter wheel, 3-position slider for dual camera
Filter Unit DAPI EM 445nm/50nm   |  GFP/ FITC EM 530nm/50nm 
DsRed / TRITC EM 605nm/60nm |   Cy5 EM 695nm/40nm
Eyepiece WF 10X/23 plan eyepiece, high eye point; centering telescope, 45°inclined, interpupillary distance adjustment 50‒75mm, adjustable diopter
Objective Converter Converter with five-hole internal positioning; Ball bearing for internal position ning
Stage Manual: 240mm× 260mm fixed stage; Range of movement: 135mm×85mm
Motorized: Minimum step size: 50nm; Stroke: X:114mm Y:75mm;Repeatability:±0.1um;
Maximum speed: ≥100mm/s;Stage size: ≥270x170mm
Maximum load capacity>1KG (Horizontal)
Z Driver Focusing resolution minimum step size: 0.05μm; Repeatability ±0.2μm; Maximum stroke: 10mm
Focusing Mechanism Coaxial coarse/ fine adjustment with limit and locking devices,
 Low level coaxial focusing handwheel ; Handwheel graduations of fine adjustment:1μm
Software Feature Multi-color fluorescence localization processing;Z-stack data processing; Large image stitching;
Image analysis; Imaging data management;3D imaging constrution
Recommended Installation Conditions Temperature 23 ± 5˚C, Humidity 70% RH or less
(no condensation)
Temperature 23 ± 5˚C, Humidity 70% RH or less
(no condensation)
Weight 11.0 Kg (24.3 lbs) 26.0 Kg (57 lbs)
Size 11.2 (W) x 15.9 (L) x 8.9 (H) inches |
286.7 (W) x 405.1 (L) x 226.5 (H) mm
14 (W) x 23.9 (L) x 8.9 (H) inches |
357.0 (W) x 606.0 (L) x 225.0 (H) mm

 


 

SpinDisk Confocal Microscopy Acquistion Atlas

 

High-Definition Visual Assets: Should you require high-definition authentic images and video footage, we invite you to get in touch with us.

 

Mouse nerve 3D tomography, 20X objective lens, scanning depth ~100 microns

(Original Image)

Mouse nerve tomography 3D reconstruction, 20X objective lens, scanning depth ~100 microns

Mouse nerve 3D tomographic scanning, 60X objective lens, scanning depth ~100 microns

(Original Image)

Mouse nerve tomography 3D reconstruction, 60X objective lens, scanning depth ~100 microns

(The picture has been processed with background noise reduction)

   

Zebrafish 3D tomography (Microscope system uses Olympus IX83)

Cell mitosis CETN1-GFP green, SiR-DNA blue (Microscope system uses Nikon Ti2)

BPAE cells with MitoTrackerTM Red CMXRos, Alexa FluorTM 488 Phalloidin, and DAPI

BPAE cells, DAPI, 60X ojective Lens (Original Image)

 

BPAE cells with MitoTrackerTM Red CMXRos, Alexa FluorTM 488 Phalloidin, and DAPI

BPAE cells, FITC, 60X ojective Lens (Original Image)

 

BPAE cells with MitoTrackerTM Red CMXRos, Alexa FluorTM 488 Phalloidin, and DAPI

BPAE cells, CY5, 60X ojective Lens (Original Image)

 

BPAE cells with MitoTrackerTM Red CMXRos, Alexa FluorTM 488 Phalloidin, and DAPI

BPAE cells, DAPI + FITC + CY5, 60X ojective Lens (Original Image)

 

BPAE cells with Mouse Anti-α-tubulin, Alexa FluorTM 488 Phalloidin, and DAPI

BPAE cells, DAPI, 60X ojective Lens (Original Image)

 

BPAE cells with Mouse Anti-α-tubulin, Alexa FluorTM 488 Phalloidin, and DAPI

BPAE cells, FITC, 60X ojective Lens (Original Image)

 

BPAE cells with Mouse Anti-α-tubulin, Alexa FluorTM 488 Phalloidin, and DAPI

BPAE cells, CY5, 60X ojective Lens (Original Image)

 

BPAE cells with Mouse Anti-α-tubulin, Alexa FluorTM 488 Phalloidin, and DAPI

BPAE cells, DAPI + FITC + CY5, 60X ojective Lens (Original Image)

 

BPAE cells1-100X-SpinDisk-405&488&525

BPAE cell 1, 100X objective lens, Laser 405,488 & 525

 

(Original Image)

BPAE cells2-100X-SpinDisk-405&488&525

BPAE cell 2, 100X objective lens, Laser 405,488 & 525

 

(Original Image)

Cyto-Golgi scan, 100X objective lens

(Original Image)

Cyto-Golgi scan, 100X objective lens

(Original Image)

   

Hippocampal_coronal_slice, 60micron thickness

 
   

Mouse neural tomography, 10X objective lens, wide field effect

(Original Image)

Mouse nerve tomography, 10X objective lens, turntable effect

(Original Image)

   

Mouse nerve tomography, 40X objective lens, wide field effect

(Original Image)

Mouse nerve tomography, 40X objective lens, turntable effect

(Original Image)

Mouse nerve tomography, 60X objective lens, multi-image superimposed effect in Z direction, superimposed depth ~100 microns

 

Mouse neural tomography, 100X objective lens

( Original Image)

Spinach rhizome, 20X objective lens

(Original Image)

Plant leaf stems, 20X objective lens

(Original Image)

 

 

Sago palm leaf, 20X objective lens

(Original Image)

Wheat seeds, 20X objective lens

(Original Image)

 


Parameters

Basic

P Series Box

Basic

P Series

Advance

P Series

Basic

L Series

Advance

L Series

SpinDisk

Basic

SpinDisk

 Advance

SIM Basic SpinDisk SIM
Image Frame Rate 4fps@512x512 4fps@512x512 4fps@512x512 10fps@1024x1024 14fps@1024x1024 100fps@2048x2048 100fps@2048x2048 13fps@1024x1024 13fps@1024x1024
Resolution ~230 nm  ~230 nm  ~230 nm  ~230 nm  150-200nm ~230 nm  ~230 nm  ~100 nm  ~100 nm 
Image Depth <100µm <100µm <100µm <100µm <600µm <200µm <200µm <50µm <200µm
No. of Laser  1 1 4 1 4 4 4 4 4
Wavelength Choice 

405/445/488

/525/561/640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

Detectors Detector Detector Detector CMOS Camera sCMOS Camera sCMOS Camera Dual sCMOS Camera(Single sCMOS optional) sCMOS Camera Dual sCMOS Camera(Single sCMOS optional)
Microscope  Non Inverted (Upright Customizeable) Inverted (Upright Customizeable) Inverted (Upright Customizeable) Inverted (Upright Customizeable)

Inverted or

Upright 

Inverted or

Upright 

Inverted or

Upright 

Inverted
Stages Non

Z Motorized

XY Manual

XYZ Motorized  Z Motorized
XY Manual
XYZ Motorized  XYZ Motorized  XYZ Motorized  XYZ Motorized  XYZ Motorized 
Electronics system     Motorized Filter
Motorized Pin hole
  Motorized Filter Motorized Filter Motorized Filter Motorized Filter Motorized Filter
image contrast Medium Medium High Medium High High High High High
Upgrade option Non Non Upgrade to 30fps high Speed  Non Non Non Upgrade to SPIN Disk SIM 100nm resolution Upgrade to SPIN Disk SIM 100nm resolution Non
Customized Option     Yes   Yes Yes Yes    
Applications Biomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning; Biomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning;
Biomedicine: three-dimensional fluorescence microscopy imaging observation of cells and tissues, multi-channel fluorescence detection
Reflective 3D microscopic imaging, surface shape detection,   rapid analysis and measurement of materials and microelectronic device   surface shape Fast 3D imaging of thick tissue, 3D histopathological image   detection, 3D histomorphological research of small animal tissues and organs   such as brain neurons, liver, kidney, etc. Dynamic fluorescence imaging of living cells/tissues,   microscopic observation of small animals such as zebrafish and nematodes Dynamic fluorescence imaging of living cells/tissues,   microscopic in vivo observation of small animals such as zebrafish and nematodes, and in vivo observation of multi-channel fluorescent organelles. Dynamic fluorescence super-resolution imaging of living cells   can be used for live cell dynamics, allowing tracking of biological changes   at cellular and subcellular levels Dynamic fluorescence super-resolution imaging of living cells,   3D imaging of tissues, organoids, spheroids and small organisms.

 

Wide Field Raman Microscope Fluorescence / Photoluminescence Microscope Time Correlated Single Photon Counting (TCSPC) For SPAD Testing 

 

XY Microscope Piezo Stages
Model ST-XY3-11075 ST-XY2-7550
Image    
Stroke(mm) 110 X 75 75 X 50
Driver Piezoelectric Motor Piezoelectric Motor
Maximum Load Capacity(KG) 1 1
Maximum Speed(mm/s) 100 100
Minimum Step Size(nm) 20 (50, 100nm for option) 50
Repeatability(nm) +/-200 +/-200
Position Sensor Grating Grating
Material Aluminum Alloy Aluminum Alloy
Weight(KG) 3.3 2.5

 

 

Parameter ST4LM
Image
Wavelength 405/488/525/638mm
Center wavelength ±5 nm
Output Power >500mw
Output Mode SMA905, Core 400um, 0.39NA, Armor Fiber
Laser Type CW laser
Monochromacies <10 nm
Power Stability <2%
Laser Power Adjustment Accuracy  1%
Models Optoelectronic all-in-one machine
Software Control RS232
Software SDK Support secondary development of SDK or serial port protocol. Provided operating software, SDKs, dependency libraries, etc

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Compare Model Drawings & Specs Availability Reference Price
(USD)
SpinDisk Basic Spinning Disk Confocal Microscope
Spinning Disk Confocal Microscope, SpinDisk Basic Series, Image frame rate 100fps@2048x2048, Resolution ~230nm, image depth <200um, No. of Laser - 4, Wavelength choice 405/445/488/525/561/640nm, sCMOS Camera detectors, Inverted or upright microscope, XYZ motorized stages, high image contrast, customized option
6-10 Weeks Request for quote
SpinDisk Advance
Spinning Disk Confocal Microscope, SpinDisk Advance Series, Image frame rate 100fps@2048x2048, Resolution ~230nm, image depth <200um, No. of Laser - 4, Wavelength choice 405/445/470/520/528/555/640nm, Dual sCMOS Camera detectors (single sCOMS optional), Inverted or upright microscope, XYZ motorized stages, Motorized filter electronics system, high image contrast, upgrade to SpinDisk SIM 100nm resolution, customized option
6-10 Weeks Request for quote

SpinDisk Advance - Parameter

SpinDisk Basic Spinning Disk Confocal Microscope - Parameter

SpinDisk Advance - Download

SpinDisk Basic Spinning Disk Confocal Microscope - Download

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