Laser Line Scan Confocal Microscope | SIMTRUM Photonics Store

Unlike traditional confocal microscopy, where images are acquired pixel by pixel in a raster scan pattern, a line-scan confocal microscope captures an entire line of information simultaneously. This dramatic reduction in acquisition time is ideal for observing fast-moving objects, dynamic cellular processes, and live biological specimens with minimal motion artifacts.

The heart of the high-speed line-scan confocal microscope is its ability to scan laser light or illumination across a sample along a line, while simultaneously collecting emitted fluorescence or reflected light from that same line. This synchronized approach not only provides rapid image acquisition but also minimizes out-of-focus light and enhances image contrast, similar to conventional confocal microscopy.

Product Brochure Link: 

The introduction of high-speed line-scan confocal microscopy has opened up new frontiers in research, offering a window into the previously inaccessible world of fast biological and materials events. Its ability to provide detailed, time-resolved images with minimal photobleaching and phototoxicity makes it a valuable tool for scientists and researchers across a wide range of disciplines. 

Furthermore, our XY Microscope Piezo Stages has been designed for use our SIMSCOP Series Microscopes to provide motorized XY positioning of microscopy  samples. Access for SIMTRUM's XY Microscope Stages can be click here.

Features & Benefits

• High-speed acquisition (50fps@1024x1000 pixels)
• Strong optical sectioning
• Single molecule imaging
• Multi-color fluorescence (Max. 4 channels)
• High sensitivity
• Deep imaging module(Max. depth:600µm)
• Large field of view(60X: 0.36mm, 40X: 0.54mm)
• Low photobleaching and phototoxicity
• Strong optical sectioning

L series Industry: Single-wavelength ordinary linear array detector, designed for simple strong fluorescence imaging and reflection imaging, used for materials science, compound fluorescence detection and surface defect detection.

L Series Research: Four-wavelength high-sensitivity back-illuminated CMOS detector, designed for biological weak fluorescence imaging and used for the study of life science cells and pathological tissues. The depth module can be used for in vivo imaging of thick tissue and small animals, targeting major research projects in biomedical research institutes and users of public instrument platforms, as well as medical laboratory testing institutions.

 Left: Wafer(10X) Right: Calcium Titanium Quantum Dots (10X)

     3-day post-fertilized zebrafish heart labelled by EGFP

High-speed Acquisition (50fps@1024x1000 pixels)

Due to the different scanning methods, the capture speed of line scan copolymerization is at least 100 times faster than that of conventional confocal technology, and the phototoxic reaction and photobleaching reaction are low. It is not only the best choice for imaging living cells and tissues, but also very suitable for fixation. Rapid volumetric collection of samples, even from small live animals.

Large FOV & High Sensitivity

With a large usable field of view (FOV), our scientific-grade CMOS cameras offer up to a 5.5-megapixel sensor with both a 60x objective lens (0.36mm) and a 40x objective lens (0.54mm) for maximum field of view. Maximizing the field of view of fluorescence microscopy is becoming increasingly important in a wide range of applications, including high-content scanning of large areas of cells, imaging of developing embryos, neuronal mapping, and tissue imaging.

Deep Imaging Module (Max. imaging depth: 600µm)

Wafer defect inspection system detects physical defects (foreign substances called particles) and pattern defects on wafers and obtains the position coordinates (X, Y) of the defects. 

Using line-scan confocal technique can speed up the inspection time by more than 10 times

Upgrade to Fluorescence lifetime imaging microscopy (FLIM) 

FLIM is a type of microscopy that allows for the visualization and analysis of biological samples based on the fluorescence lifetime of the fluorophore being used. FLIM measures the time between the excitation and emission of photons in a sample, which can provide information about the properties of the fluorophore and the environment in which it is located.

FLIM can be used to study a wide range of biological processes, including protein-protein interactions, enzyme activity, and ion concentration changes. It is often used in combination with other imaging techniques, such as confocal microscopy, to provide more detailed information about the sample.

Upgrade to Single / Two /Multi Photon Microscope

In two-photon microscope, a laser emits light at a specific wavelength that is absorbed by the fluorescent molecules in the sample. When two photons of this light are absorbed simultaneously, they provide enough energy to excite the fluorescent molecule and cause it to emit light at a longer wavelength, which can be detected by the microscope. Because two photons are required to excite the molecule, the probability of fluorescence emission is low and only occurs at the focal point of the microscope, allowing for high-resolution imaging and greater depth than conventional microscopes.

Two-photon microscopy has a number of applications in neuroscience, biology, and biomedical imaging. For example, it has been used to study the activity of individual neurons in the brain, visualize the structure and function of blood vessels, and track the behavior of cells in living tissues.

Upgrade to Confocal Spectral Microscope (Near IR I/II Confocal)

● Upgrade to Confocal Spectral Microscope (NIR I/II confocal)

● Wavelength Range UV to NIR (200nm-2.5nm)

● Spectral resolution up to 0.2nm

● Large NA setup for high-sensitivity application

Details Click here

Upgrade to Terahertz Confocal Microscope System

● 100GHz, output power: 80mW

● Spatial resolution 150-200um

The terahertz confocal microscope uses a focused beam of terahertz radiation to scan the sample being analyzed. This beam is then reflected back and collected by a detector, which creates an image of the sample based on the intensity of the reflected radiation. By using a confocal design, this microscope can achieve high resolution and can selectively focus on different depths within a sample.

 it can be used to study the microstructure and properties of materials, such as polymers, ceramics, and semiconductors, and to detect defects or anomalies in their structures. In biology and medicine, it can be used to image and study biological tissues, including skin, teeth, and cartilage, which are transparent to terahertz radiation.

Upgrade to Super Resolution Confocal Re-scan Structure illumination Microscope

A "re-scan" confocal microscope is a type of confocal microscope that uses a rapidly moving mirror or scanner to scan the laser beam across the sample multiple times, producing even higher resolution and better contrast images than standard confocal microscopes.

Overall, re-scan confocal microscopes are very powerful tools for studying biological tissues, cells, and other samples, and are widely used in research labs, medical facilities, and other scientific settings

Compatible with SIMTRUM Cryostat to perform Low-temperature Raman measurements -190 to 600 degrees

● 8 probe arm able to upgrade to adjustable probe arm

● Reflection or transmission mode available

405/445/488

/525/561/640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

Inverted or

Upright 

Inverted or

Upright 

Inverted or

Upright 

Z Motorized

XY Manual

Don't have time to search the products one by one? No worries. you can download the full range of SIMTRUM Product Line Cards.

Click it now.

Want to stay closer to the Market Dynamics and Technological 

Developments? Just take 5 seconds to Sign In as a member of 

SIMTRUM, we will bring you the most up-to-date news. 

(Sign in button on the top right of the screen).