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Laser Line Scan Confocal Microscope | SIMTRUM Photonics Store

Laser Line Scan Confocal Microscope
SIMSCOP L Series LFMM (Line-scan Focal Modulation Microscopy)

LSFMM (Line-scan Focal Modulation Microscopy) is a high-speed, high-contrast multi-dimensional imaging platform capable of three key imaging advantages. At its core is a focal modulation module for high-contrast imaging. It significantly reduces the background signal caused by multiple scattering and effectively picks up the high-resolution signal related to the ballistic excitation light. Consequently, the signal to background ratio and the spatial resolution can be maintained to a deeper penetration depth, which is about two to four times deeper than conventional confocal microscopes. High acquisition speed is the second feature of LSFMM. Capturing at speeds at least 100x faster than conventional confocal technology, LSFMM is the optimal solution for live cell and tissue imaging, providing low phototoxicity and photobleaching, or perfect for fast volume acquisition of fixed samples and even small live animals.


                                         

                                                                   

The third characteristic is large field of view (FOV) available. Our scientific CMOS camera can offer up to 5.5 Megapixel sensor, yielding the largest available field of view with 60x objectives (0.36 mm) and 40x objectives (0.54 mm). Maximizing view in fluorescence microscopy is of increasing relevance across a wide range of applications, including high content screening of large fields of cells, imaging of the developing embryo, neuron mapping and tissue imaging.


Features & Benefits

√ High-speed acquisition

√ Strong optical sectioning

√ Single molecule imaging

√ Multi-color fluorescence

√ High sensitivity

√ Large field of view

√ Low photobleaching and phototoxicity

√ Super-resolution

√ Intuitive software



Technical Features Explained

LSFMM (Line-scan Focal Modulation Microscopy) is a novel microscopy method that has been developed to achieve a deeper penetration depth on the basis of confocal microscopy. Equivalently selective excitation is achieved by modulating the light intensity at the focal point only. Fluorescence emission or backscattered light are collected and demodulated. As a result, only focal signals are decoded and thus significantly reducing the background signals. Specially, we add a cylindrical lens to generate a line-focus at the sample and achieve line-scan imaging. Therefore, the imaging acquisition speed is improved significantly compared with conventional point-scan confocal microscopes.


                                                                                   
















                                                      Chicken chondrocytes labelled with a lipid tracer


     3-day post-fertilized zebrafish heart labelled by EGFP



Comparison with other conventional confocal microscope models
ParametersAdvance L SeriesOlympus FV3000Nikon C2+ZEISS LSM 980LEICA TCS SP8
Typical frame rateUp to 140 fps1.8 fps2 fps13 fps7 fps
Imaging depthUp to 600 μmtypically 50-200  μm
FMM contrast enhancement20-30 dBNo
Noise level1.5 photons17 photons at 10 µs pixel time;
7.6 photons at 2 µs pixel time



Product specifications and Brochures

Product Brochure Link: 


The Product Configurations Comparison

Hardware FeatureHardware FeatureBasic L SeriesAdvance L Series
High-speed line- scan laser confocal imagingUp to 140 fps for fast cell dynamics (small ROI).At least 100   x faster than conventional confocal
FMM image contrast enhancementImaging deeper in cells and tissue At least 2-4 times deeper   than conventional confocal 20-40 dB enhancement in image signal-to-background   ratio-
Large field of viewCapture more in a single image.
    Matches large sCMOS sensors
-
Low noise levelAcquire noise-free images with weak fluorescence-
16-bit dynamic rangeCapture both weak and bright signals without saturation
Multi-color fluorescence imagingChoice of 4 wavelengths up to 640 nm Any two colours   simultaneously - match penetration depth of two labelled targets instantlyOpt. to choose laser
Super-resolutionAcquire higher resolution images than the diffraction limit by   algorithm-
XY-motorized stageAcquire stitching images automatically-
Z-motorized stageAcquire volumetric images automatically


Software FeatureBenefits

Automatical image acquisition 

GUI software

·   Microscope configuration, image acquisition, 3D images visualization and rendering.

·   Immediate visual feedback on experimental progress to evaluate data and make appropriate 

    decisions in real-time

Imaris file format·   Easy transfer of data to Imaris for comprehensive downstream multi-dimensional analysis


Technical Data

ParametersBasic L SeriesAdvance L Series
Laser combiner488nm (original configuration);   Laser wavelengths are optional according to user requirement.405nm, 488nm, 561nm, 640nm   (original configuration); Laser wavelengths are optional according to user   requirement.
Laser powerMaximum 20mW fiber input
Frame rate10 fps (1024 x 1024   pixels)
    100 fps (1024 x 100 pixels)
    Fast scan mode
14 fps (1024 x 1024   pixels)
    140 fps (1024 x 100 pixels)
    Fast scan mode
Image resolution100 x 100 pixels to 1920 x 1920   pixels100 x 100 pixels to 2048 x 2048   pixels
Image format8/16 bit8/16 bit
Noise level6.2e-0.9e-
Noise level (count in photons)8.8 photons1.5 photons
Lateral resolutionoptical diffration limit1.2-1.4 fold over optical diffration limit
Number of laser channel41
Microscope stageSemi-motorized stage with piezo z scannerFully-motorized and automated stage with piezo XYZ  scanner



Creating the Optimum Products for you


Step 1  Select the Scan Box You Require

                     → Basic L Series

             → Advance L Series


Step 2  Choose Laser Combination for your Selected Model

Many combinations of the following laser lines can be supported. For specific laser wavelength configurations please Click here to speak to our Sales Engineer.

Available Wavelengths (nm)Power (mW)
405>20
445>20
488>20
514>20


Available Wavelengths (nm)Power (mW)
532>20
561>20
640>20
785>20



Step 3  Select the Inverted Microscope Model

Recommended Microscope Models


OlympusNikonZeiss& LeicaMshot
Olympus IX73Nikon Ti-EZeiss AxioObserverMF53N
Olympus IX83Nikon Ti-ULeica DMi6000

Nikon Ti2-ELeica DMi8

Nikon Ti2-A


Nikon Ti2-U



Step 4  Select the Required Accessories

Please discuss any additional requirements, such as motorized XY and Z stage control, incubation and accessories for your specific application needs with our Sales Engineer.



Applications

Onion Cell Imaging (20x/0.45)


Thick Beads Phantom Imaging (20x/0.45)
Routine Confocal
Advance L Series

Routine Confocal                                    Advance Series




Mouse Brain GFP Neurons (20x/0.95, 3D projection)



Mouse Epithlial Cells (40X/0.75, DAPI/FITC)



Parameters

Basic

P Series Box

Basic

P Series

Advance

P Series

Basic

L Series

Advance

L Series

SpinDisk

Basic

SpinDisk

 Advance

SIM BasicSpinDisk SIM
Image Frame Rate4fps@512x5124fps@512x5124fps@512x51210fps@1024x102414fps@1024x1024100fps@2048x2048100fps@2048x204813fps@1024x102413fps@1024x1024
Resolution~230 nm ~230 nm ~230 nm ~230 nm 150-200nm~230 nm ~230 nm ~100 nm ~100 nm 
Image Depth<100µm<100µm<100µm<100µm<600µm<200µm<200µm<50µm<200µm
No. of Laser 114144444
Wavelength Choice 

405/445/488

/525/561/640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

DetectorsDetectorDetectorDetectorCMOS CamerasCMOS CamerasCMOS CameraDual sCMOS Camera(Single sCMOS optional)sCMOS CameraDual sCMOS Camera(Single sCMOS optional)
Microscope NonInverted (Upright Customizeable)Inverted (Upright Customizeable)Inverted (Upright Customizeable)Inverted (Upright Customizeable)

Inverted or

Upright 

Inverted or

Upright 

Inverted or

Upright 

Inverted
StagesNon

Z Motorized

XY Manual

XYZ Motorized Z Motorized
XY Manual
XYZ Motorized XYZ Motorized XYZ Motorized XYZ Motorized XYZ Motorized 
Electronics system

Motorized Filter
Motorized Pin hole

Motorized FilterMotorized FilterMotorized FilterMotorized FilterMotorized Filter
image contrastMediumMediumHighMediumHighHighHighHighHigh
Upgrade optionNonNonUpgrade to 30fps high Speed NonNonNonUpgrade to SPIN Disk SIM 100nm resolutionUpgrade to SPIN Disk SIM 100nm resolutionNon
Customized Option

Yes
YesYesYes

ApplicationsBiomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning;Biomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning;
Biomedicine: three-dimensional fluorescence microscopy imaging observation of cells and tissues, multi-channel fluorescence detection
Reflective 3D microscopic imaging, surface shape detection,   rapid analysis and measurement of materials and microelectronic device   surface shapeFast 3D imaging of thick tissue, 3D histopathological image   detection, 3D histomorphological research of small animal tissues and organs   such as brain neurons, liver, kidney, etc.Dynamic fluorescence imaging of living cells/tissues,   microscopic observation of small animals such as zebrafish and nematodesDynamic fluorescence imaging of living cells/tissues,   microscopic in vivo observation of small animals such as zebrafish and   nematodes, and in vivo observation of multi-channel fluorescent organelles.Dynamic fluorescence super-resolution imaging of living cells   can be used for live cell dynamics, allowing tracking of biological changes   at cellular and subcellular levelsDynamic fluorescence super-resolution imaging of living cells,   3D imaging of tissues, organoids, spheroids and small organisms.



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Compare Model Drawings & Specs Availability Reference Price
(USD)
Basic L Series
Laser Line Scan Confocal Microscope Basic L Series, Image frame rate 10fps@1024x1024, Resolution ~230nm, image depth <100um, No. of Laser - 1, Wavelength choice 405/445/488/525/561/640nm, CMOS Camera detectors, Inverted microscope(upright customizable), Z motorized / XY Manual stages, Medium image contrast
6-10 Weeks Request for quote
Advance L Series
Laser Line Scan Confocal Microscope Basic L Series, Image frame rate 14fps@1024x1024, Resolution 150-2000nm, image depth <600um, No. of Laser - 4, Wavelength choice 405/488/561/640nm, sCMOS Camera detectors, Inverted microscope(upright customizable), XYZ motorized stages, High image contrast, Motorized filter Electronics system.
6-10 Weeks Request for quote

Advance L Series - Parameter

Basic L Series - Parameter

Advance L Series - Download

Basic L Series - Download

Accessories

Compare Model Drawings & Specs Availability Reference Price
(USD)