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SIMTRUM-Confocal and Raman Microscope

Products / Microscopes /Confocal Microscope /Confocal and Raman Microscope /
Confocal and Raman Microscope
Overviews Specifications Point scanning imaging Raman Model

SIMSCOP Confocal And Raman Microscope——Nano PLUS Product Overview

The SIMSCOP Confocal And Raman Microscope——Nano PLUS is a cutting-edge scientific research platform integrating high-resolution optical imaging and Raman spectroscopy analysis, specifically designed for the ultra-high spatial resolution and chemical specificity required in life sciences, semiconductor failure analysis, and advanced materials research[cite: 10]. The system natively supports smooth switching between multiple excitation wavelengths: 405nm, 532nm, 633nm, and 785nm, and when combined with highly sensitive photomultiplier tubes (PMT) and a scientific-grade spectrometer, it not only enables high-speed fluorescence scanning imaging but also simultaneously acquires the Raman spectral characteristics of the sample, achieving precise in-situ mapping of physical morphology and chemical composition.

SIMSCOP Confocal Dual-Modal Microscope Nano PLUS Appearance

Key Specifications (Imaging)

  • • Lateral Resolution: 230nm@100x Oil objective
  • • Imaging Depth: < 100um
  • • Fluorescence Lasers: 405/488/561/640nm
  • • Detection Range: 400-700nm
  • • Scan Speed: Up to 8 fps@512x512; 16 fps@256x256
  • • Field of View (FOV): Φ18mm inscribed square
  • • Fluorescence Detection Efficiency: Peak photon detection efficiency up to 41%

Key Specifications (Raman & Hardware)

  • • Raman Laser: Single-mode 532nm laser
  • • Laser Power: 100mW
  • • Maximum Detection Wavenumber: 200-4850cm-1
  • • Minimum Wavenumber Resolution: 5.5~7cm-1
  • • Raman Detector Type: Cooled BSI CCD
  • • Stage Resolution: Lateral <1um, Axial 30nm

SIMSCOP Confocal Dual-Modal Microscope — Nano PLUS Specifications

Module Parameter Nano PLUS
Multi-
channel
Fluorescence
Confocal
Module		 Laser Type Standard Wavelengths: 405±5 nm; 488nm±5nm; 561nm±1nm; 640nm±5nm
Single Wavelength Output Power: > 20mW, Power Stability: < 1%, Power Adjustment Accuracy: 0.1%
TTL Modulation: 1kHz, Linewidth: < 3nm, Output Coupling: Single-mode polarization-maintaining (PM) fiber coupling (TEM00)

Optional Wavelengths: 375/445/473/515/525/532/633/660/685/785/808nm
Optional Modulation: AOTF/AOM; ≥30mW per line
Optional Power: High-power version up to 50mW class
Confocal Point Scanning Head Modular scanning head (detachable for maintenance); Integrated scanning/detection directional design
Arbitrary combinations of point scanning, rotation, and X-Y-Z-t-λ multidimensional scanning; Supports 5D image acquisition and display (multi-channel fluorescence imaging, time series scanning, multi-position acquisition, Z-stacking, and tile stitching)
Additional spectral detection port adaptable for spectroscopy and Raman detection
Max Scanning Resolution ≥4096×4096 standard; Hardware/transmission reserved for 8192×8192
Scanning Speed (Typical) 8 fps@512x512; 16 fps@256x256
Scanning Field of View (FOV) φ18mm inscribed square
Pinhole / Aperture Fixed pinhole (optional size: 10-100 μm);
Motorized pinhole (optional): Ø0 - Ø11.5 mm; Minimum step size: 0.01 mm
Detector Peak Photon Detection Efficiency (PDE): 41%, Spectral Response: 300~950 nm,
Adaptable and upgradable to various detector types
2 detection channels (upgradable to 4 channels)
Detection Bands 400-700 nm; 4-channel dichroic mirror: 405/488/561/640; 6-position motorized filter wheel,
Emission filters: 452nm/45, 530nm/43, 607nm/36, 685nm/40*, Blocking efficiency: OD≥6
Image Bit Depth 16 bit
PLUS
Raman
Module		 Laser Single-channel laser: 1 channel
Standard single wavelength: 532 nm
Power: 100 mW, Power stability: 1%, Linewidth: 0.003 nm, SM fiber
Optional wavelengths: 405 nm, 633 nm, 785 nm, 1064 nm
Spot Size ~1 μm @50x
Raman Parameters Max Spectral Range: 200-4850 cm-1
Min Spectral Resolution: 5.5~7 cm-1 (@ 25 μm Slit)
Numerical Aperture: F/4
Signal-to-Noise Ratio (SNR): 1000:1
Integration Time: 6 ms - 60 mins
Upgradable to High-Sensitivity Raman Pro, sensitivity enhanced by 9.5× (refer to test chart)
Detector Parameters Cooled BSI CCD
Pixels: 1044×64
Pixel Size: 24×24 μm
Cooling Temperature: -20℃ (Upgradable to deep cooling type -70℃)
Widefield Camera Back-illuminated CMOS camera: 15 fps@5440×3648; 50 fps@2736×1824
Detection Mode Standard: Widefield Raman
Optional: Confocal Raman (fixed pinhole or tunable pinhole), Confocal Resolution: ~200 nm @100x objective
Optional
Upright /
Inverted
Microscope		 Microscope Platform Fully motorized fluorescence microscope:
Infinity optical system; 45 mm parfocal distance; Motorized 6-hole objective nosepiece
Fluorescence Unit Optional fluorescence module for microscope
Transmitted Köhler Illumination Köhler illumination with 100W long-life halogen lamp
Epi-illumination LUMOS high-brightness LED fluorescence light source (Optional mercury lamp or metal halide lamp)
Motorized LWD Condenser Abbe condenser N.A. 0.90 (Optional turret phase contrast / darkfield condenser, LWD N.A. 0.65 WD10.7 condenser, swing-out condenser)
Nosepiece / DIC Slider Coded / motorized 6-hole objective nosepiece
4X phase contrast ring slider (0.55, Ph0), 10X/20X/40X phase contrast ring slider (0.55, PH1)

Imaging Module

Simultaneous Multi-Channel Imaging & Widefield Comparison
Widefield vs Confocal Comparison (Mouse Brain Neurons 40X)
Fig: Widefield vs. Confocal Imaging Comparison (Mouse Brain Neurons 40X)

Up to 4 detector channels for simultaneous imaging to achieve higher acquisition speeds:
You can utilize different fluorophores or fluorescent probes with distinct emission spectra and capture their pure signals concurrently. This is particularly suitable for simultaneously investigating multiple targets or molecules within a specimen without sequential scanning, thereby effectively minimizing phototoxicity and photobleaching.

Confocal Optical Architecture & External Detection Expansion
Nano PLUS Point Scanning Confocal Optical Layout

Integrating 405nm / 488nm / 561nm / 640nm multi-wavelength laser coupling with a high-speed 2D Galvo scanner, along with dedicated fiber-coupled ports to support external detection extensions including Raman spectrometers, fluorescence spectrometers, photon counting modules, and TCSPC.

Flexible Detector Options (GaAsP PMT / MA PMT / SiPM)

The flexibility to choose detector types allows you to optimize the signal-to-noise ratio (SNR) based on specific experimental requirements. Combining multiple detectors with functional data enables accurate quantitative analysis of fluorescence intensity, emission spectra, and fluorescence lifetime measurements, comprehensively supporting quantitative biology and materials science research.

Detector Parameter SiPM GaAsP PMT Multialkali PMT Ultra Bialkali
PMT
Active Area 6mm × 6mm Ø5 mm Ø8 mm Ø8 mm
Spectral Range (nm) 200 – 900 300 – 740 185 – 870 230 – 700
Peak Wavelength (nm) 420 520 400 400
Dark Current (nA) 900 3 1 1
Peak Detection Efficiency 38% @ 420nm 45% @ 520nm 23.87% @ 400nm 40.3% @ 400nm
Rise Time 180 ps 1 ns 0.57 ns 0.57 ns
 

SIMSCOP Confocal Dual-Modal Microscope — Nano PLUS Raman Module Overview

Building upon high-performance point-scanning confocal fluorescence imaging, the Nano PLUS Confocal Dual-Modal Microscope seamlessly integrates a professional-grade Raman spectroscopy module. Breaking through the limitations of conventional single-modal systems, it consolidates high-resolution microscopic imaging, high-sensitivity micro-Raman spectral acquisition, chemical mapping, and multi-dimensional scanning analysis onto a unified platform, pushing the feasibility boundaries for advanced research in life sciences, materials discovery, and semiconductor inspection.

Raman Module Key Highlights
• Ultra-High Raman Sensitivity: High-throughput optical design combined with a deep-cooled detector provides up to a 10× boost in detection sensitivity.
• Upright / Inverted Microscope Compatibility: Flexibly accommodates living cell culture dishes, glass slides, patterned semiconductor wafers, and bulk materials.
• Multi-Wavelength Laser Options: Standard 532 nm excitation, with optional modular expansion across 405 nm / 633 nm / 785 nm / 1064 nm lines.
• Customized In Situ Stages: Broad support for tensile stages, heating/cooling stages, in situ electrochemical cells, vacuum chambers, and electrical probe stations.
• Non-Coaxial Optical Measurements: Transcends traditional coaxial constraints, expanding versatility for exciton transport and advanced nanophotonics experiments.
High Sensitivity Raman Performance Test
Sensitivity Benchmark: Equipped with a high-sensitivity spectrometer system, achieving substantial gains in SNR and acquisition speed.
 
Optical Layout & Modular Architecture
Nano PLUS Raman Module Optical Diagram
Fig: Schematic Layout of the SIMSCOP Nano PLUS Raman Dual-Modal Confocal Optical System
Optical Design Highlights: Integrates multi-channel laser collimation and filtering modules, widefield color camera, high-NA objective, and precision motorized XYZ stage. Precise coupling between tube lens and spectrometer interfaces ensures diffraction-limited microscopic observation while maximizing Raman collection throughput.
 
Typical Application Fields

Combining diffraction-limited spatial resolution with fingerprint Raman spectral identification, the Nano PLUS Raman Module serves key research disciplines:

• Biology & Life Sciences Cellular metabolic dynamics, tissue slice spectral fingerprinting, and targeted intracellular drug tracking.
• Materials Science & Nanomaterials 2D crystal layer characterization, phase identification, local crystallinity, and structural defect mapping.
• Graphene & Carbon Nanotubes Precise D/G/2D peak analysis, chirality assignment, and localized strain/stress distribution mapping.
• Catalysis & Energy Chemistry In situ monitoring of catalytic pathways, active phase transitions, and electrode interfacial evolution.
• Semiconductors & Microelectronics Micro-area stress mapping, dopant homogeneity screening, defect localization, and failure analysis.
• Pharmaceutical QA & Contamination Analysis Polymorph verification, API-excipient spatial distribution, and micro-contaminant particle screening.
 


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