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FISH Filters | SIMTRUM Photonics Store

Products / Optics /Filters /Filters for FISH /
Filters for FISH
Overview Specifications Related Product Order Now!

Fluorescence In Situ Hybridization (FISH) refers to the use of fluorescent dye-labeled single-stranded nucleic acids with known sequences as probes, through the principle of base complementarity pairing, specifically binding to the nucleic acids in the sample to be tested, and then by observing the fluorescence signal, the qualitative, quantitative and localized analysis of the nucleic acid to be detected in the sample.  FISH is widely used in prenatal diagnosis, genome structure analysis and other fields due to its rapid speed, strong signal, high specificity, and multiplex staining.

 

There are many factors that can affect FISH results, including sample preparation and handling, microscopes, light sources, fluorescent probes, and filters. FISH is typically based on a fluorescence microscope to visualize the signal, using three types of filters: excitation plates, dichroic mirrors, and emitting plates. Its working principle is shown in the following figure:

  • Excitation Tablets: select the light that transmits a certain part of the wavelength of the light source to excite the fluorescent probe;
  • Dichroic Mirror: reflects the light from the light source to the sample, transmits the generated fluorescence and reflects the scattered light of the excitation light;
  • Transmitter: Select the light that transmits a certain part of the fluorescence band to the detector or the human eye, and completely blocks the stray light of the excitation light.
In FISH experiments, the size of the filter bandwidth can have an important impact on the contrast of the FISH image. Increasing the bandwidth improves the excitation and reception efficiency, resulting in an enhanced signal reception. However, the actual received signal includes both the fluorescence signal of the probe and the background noise of the sample, and the increase in bandwidth will increase the intensity of both at the same time. The reduction of the bandwidth reduces the intensity of the background noise, but also reduces the fluorescence signal.
 
Therefore, for FISH experiments that require high contrast, there will be an optimal bandwidth for each specific dye to enable the FISH image to achieve the best contrast. The bandwidth of Chroma's FISH filters is optimized for commonly used FISH dyes to provide the best image contrast for your FISH experiments.
"B" signals 91% more than "A", but "B" is 226% more noisy than "A". "A" for better contrast!
Chroma FISH filter sets have the following features:
  • High transmittance, high cut-off depth and high spectral steepness;
  • Ensure sufficient brightness while providing the best image contrast;
  • Eliminate the effect of bleed-through;
     a,Eliminate Gold and Aqua bleed-through to the Green channel;
     b,Remove Gold and Orange bleed-through to the Red channel;
  • Special processing is applied to the DAPI channel in the multi-band filter set to avoid the DAPI signal being too strong and masking the signal of other channels;
  • Multi-color image overlay with zero drift;
  • Magnetron sputtering coating, lifetime warranty.
 

ET series - FISH multi-band filter group

DAPI is a kind of organic fluorescent dye that can bind DNA double strand specifically. In FISH experiments, DAPI is mainly used to stain and image the entire nucleus, which results in a much higher concentration of DAPI than other FISH probes. In some FISH experiment processes, DAPI is dyed at the last step, and there is no cleaning step after dyeing.

In the multi-color FISH experiment using multi-band filters, in order to avoid the signal of DAPI to mask the signal of other FISH probes, the transmission rate of the excitation plate at DAPI in the multi-band filter group of Chroma will be deliberately reduced to ensure that the DAPI signal is not too high (see the figure on the right).

Multi-band  FISH Filter set
The emission peaks of DAPI and Aqua are highly overlapping, and it is often difficult to image both probes at the same time, because a large number of DAPI signals will mask the Aqua signal.Chroma's 69014 filter set enables simultaneous observation of DAPI, Aqua, Green and Orange by reducing the excitation efficiency of DAPI. DAPI and Aqua use different excitation bands, but share the same receiving channel (see figure on the right).

69014 FISH Filter set
 
ET Series - FISH Single Band Bandpass Filter Sets
Spectral Transmission Curves  
Filter Set Excitation Tablets
(CWL/FWHM)nm		 Dichroic Mirrors
(50% cut-on)nm		 Transmitters
(CWL/FWHM)nm		 FISH Probe Remark
49000 350/50 400 460/50 DAPI  
49028 395/25 425 460/50 DAPI For 395 nm light sources
49301 405/10 425 460/36 Blue  
49302 436/20 455 480/30 Aqua  
49303 495/25 515 537/29 Green For aqua and without gold
49308 485/25 505 525/30 Green For gold and without aqua
49312 490/20 505 525/20 Green For aqua and without gold
49313 490/20 505 520/20 Green Ventanta FISH or immunofluorescence
49304 546/10 556 572/23 Gold  
49305 546/22 565 590/33 Orange For without Red
49309 539/21 556 576/31 Oange For Red
49306 580/25 600 625/30 Red For without Gold and Orange
49310 592/21 610 630/30 Red For Gold and without Orange
49311 599/13 612 632/28 Red For orange
49307 630/20 647 667/30 Far Red  
49009 640/30 660 690/50 Far Red  

ET Series - FISH Multi-band Filter Sets

Spectral Transmission Curves  

Filter Set FISH Probe
DAPI Aqua Green Gold Orange Red Far Red
59001v2 •   •        
59002v2 •       •    
59010     •     •  
590111     •   •    
590121     •   •    
59033   •   •      
69008   • •     •  
69011   • •   •    
69013v2 •   •   •    
69014 • • •   •    
69015 •   •     •  
89084v22 •   •   •    
890853 •   •     •  
1,To ensure that the Green and Orange intensities are close together, 59011 is suitable for mercury and halogen light sources, and 59012 is suitable for LED light sources;
2,Includes a dual-band excitation for simultaneous excitation of Green and Orange, a three-band excitation for simultaneous excitation of DAPI, Green and Orange, and three single-band excitations for simultaneous excitation of DAPI, Green and Orange;
3,Includes a dual-band excitation plate for simultaneous excitation of Green and Red, a three-band excitation for simultaneous excitation of DAPI, Green and Red, and three single-band excitation discs for excitation of DAPI, Green and Red, respectively.
*Note Prices do not include any shipping, tax or customs clearance charges

Dichroic beamsplitters (mirrors) are used as light guiding optics, intended to reflect a specified range(s) of wavelengths and transmit a different specified range(s) of wavelengths at a specified AOI, typically 45 degrees.  Most of Chroma’s catalog dichroics are intended for use at 45 degrees AOI, if unsure please ask us.  Dichroics are a necessary component of filter sets used in epifluorescence configurations.

Chroma manufactures UltraFlat dichroics for laser applications demanding superior levels of surface flatness, such as TIRF and other super-resolution techniques.  We offer UltraFlat dichroics either for use as individual parts or included in assembled filter sets.

However, the secret with flat dichroics is that they need to stay flat in your imaging system.  Filter cubes that use clips, springs or screws to hold dichroics will torque them out of flatness.  Chroma's TIRF & Super-resolution filter cubes keep your dichroics just as flat in your microscope as they were when we made them.

Most of our dichroics intended for more routine widefield fluorescence applications neither have nor require high levels of surface flatness but still deliver the other performance advantages of sputtered interference filter coatings.

Dichroic mirror for  FISH Single Band Bandpass Filter Sets:

Spectral Transmission Curves  

Model Wavelength (CWL): nm  Following Sets:
T400lp 400 T400lp is part of following sets:
49000 - ET/79001 - ET /49025 - ET
T425lpxr 425 T425lpxr is part of following sets:
49021 - ET /49301 - ET /49028 - ET /49053 - ET 
T455lp 455 T455lp is part of following sets:
49001 - ET /49052 - ET/49302 - ET/49032 - ET 
AT505DC 505 AT505DC is part of following sets:
39002 - AT /39009 - AT /19002 - AT /19010 - AT
T515lp 515 T515lp is part of following sets:
49003 - ET /49029 - ET/49303 - ET 
T556lpxr 556

T556lpxr is part of following sets:49304 - ET/49309 - ET 
T565lpxr 565 T565lpxr is part of following sets:
49004 - ET /49305 - ET 
AT600DC 600 AT600DC is part of following sets:
39010 - AT /19006 - AT 
T610lpxr 610 T610lpxr is part of following sets:49310 - ET
T612lpxr 612 T612lpxr is part of following sets:49311 - ET
T647lpxr 647 T647lpxr is part of following sets:49307 - ET 
T660lpxr 660 T660lpxr is part of following sets:
49006 - ET/49009 - ET /49019 - ET 

*Note Prices do not include any shipping, tax or customs clearance charges


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Compare Model Drawings & Specs Availability Reference Price
(USD)
49000 Filter Set
49000 ET Series FISH Single Band Bandpass Filter Set,FISH Probe DAPI,Filters can be purchased separately 		4-6 Weeks $725.00
49028 Filter Set
49028 ET Series FISH Single Band Bandpass Filter Set,FISH Probe DAPI,Filters can be purchased separately 		4-6 Weeks $925.00
49301 Filter Set
49301 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Blue,Filters can be purchased separately 		4-6 Weeks $925.00
49302 Filter Set
49302 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Aqua,Filters can be purchased separately 		4-6 Weeks $925.00
49303 Filter Set
49303 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Green,Filters can be purchased separately 		4-6 Weeks $925.00
49308 Filter Set
49308 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Green,Filters can be purchased separately 		4-6 Weeks $925.00
49312 Filter Set
49312 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Green,Filters can be purchased separately 		4-6 Weeks $925.00
49313 Filter Set
49313 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Green,Filters can be purchased separately 		4-6 Weeks $925.00
49304 Filter Set
49304 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Gold,Filters can be purchased separately 		4-6 Weeks $925.00
49305 Filter Set
49305 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Orange,Filters can be purchased separately 		4-6 Weeks $925.00
49309 Filter Set
49309 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Oange,Filters can be purchased separately 		4-6 Weeks $925.00
49306 Filter Set
49306 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Red,Filters can be purchased separately 		4-6 Weeks $925.00
49310 Filter Set
49310 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Red,Filters can be purchased separately 		4-6 Weeks $925.00
49311 Filter Set
49311 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Red,Filters can be purchased separately 		4-6 Weeks $925.00
49307 Filter Set
49307 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Far Red,Filters can be purchased separately 		4-6 Weeks $925.00
49009 Filter Set
49009 ET Series FISH Single Band Bandpass Filter Set,FISH Probe Far Red,Filters can be purchased separately 		4-6 Weeks $925.00
59001v2 Filter Set
59001v2 ET Series FISH Multi-band Filter Sets,FISH Probe DAPI/Green，Filters can be purchased separately 		4-6 Weeks $1050.00
59002v2 Filter Set
59002v2 ET Series FISH Multi-band Filter Sets,FISH Probe DAPI/Orange，Filters can be purchased separately 		4-6 Weeks $1050.00
59010 Filter Set
59010 ET Series FISH Multi-band Filter Sets,FISH Probe Green/Red，Filters can be purchased separately 		4-6 Weeks $1050.00
59011-1 Filter Set
59011-1 ET Series FISH Multi-band Filter Sets,FISH Probe Green/Orange，Filters can be purchased separately 		4-6 Weeks $1050.00
59012-1 Filter Set
59012-1 ET Series FISH Multi-band Filter Sets,FISH Probe Green/Orange，Filters can be purchased separately 		4-6 Weeks $1050.00
59033 Filter Set
59033 ET Series FISH Multi-band Filter Sets,FISH Probe Aqua/Gold，Filters can be purchased separately 		4-6 Weeks $1050.00
69008 Filter Set
69008 ET Series FISH Multi-band Filter Sets,FISH Probe Aqua/Green/Red，Filters can be purchased separately 		4-6 Weeks $1250.00
69011 Filter Set
69011 ET Series FISH Multi-band Filter Sets,FISH Probe Aqua/Green/Orange，Filters can be purchased separately 		4-6 Weeks $1250.00
69013v2 Filter Set
69013v2 ET Series FISH Multi-band Filter Sets,FISH Probe DAPI/Green/Orange，Filters can be purchased separately 		4-6 Weeks $1250.00
69014 Filter Set
69014 ET Series FISH Multi-band Filter Sets,FISH Probe DAPI/Aqua/Green/Orange，Filters can be purchased separately 		4-6 Weeks $1300.00
69015 Filter Set
69015 ET Series FISH Multi-band Filter Sets,FISH Probe DAPI/Green/Red，Filters can be purchased separately 		4-6 Weeks $1250.00
89084v2-2 Filter Set
89084v2-2 ET Series FISH Multi-band Filter Sets,FISH Probe DAPI/Green/Orange，Filters can be purchased separately 		4-6 Weeks $2350.00
89085-3 Filter Set
89085-3 ET Series FISH Multi-band Filter Sets,FISH Probe DAPI/Green/Red，Filters can be purchased separately 		4-6 Weeks $2350.00
T610lpxr
T610lpxr Dichroic mirror for FISH Single Band Bandpass Filter Set,Wavelength (CWL): 610 nm 		4-6 Weeks $250.00
T612lpxr
T612lpxr Dichroic mirror for FISH Single Band Bandpass Filter Set,Wavelength (CWL): 612 nm 		4-6 Weeks $250.00
T647lpxr
T647lpxr Dichroic mirror for FISH Single Band Bandpass Filter Set,Wavelength (CWL): 647 nm 		4-6 Weeks $250.00
T556lpxr
T556lpxr Dichroic mirror for FISH Single Band Bandpass Filter Set,Wavelength (CWL): 556 nm 		4-6 Weeks $250.00
T425lpxr
Dichroic beamsplitters (mirrors),Wavelength (CWL): 425 nm,Type :BS,Prices do not include any shipping, tax or customs clearance charges 		4-6 Weeks $250.00
T455lp
Dichroic beamsplitters (mirrors),Wavelength (CWL): 455 nm,Type :BS,Prices do not include any shipping, tax or customs clearance charges 		4-6 Weeks $250.00
T400lp
T400lp Dichroic mirror for FISH Single Band Bandpass Filter Set,Wavelength (CWL): 400 nm 		4-6 Weeks $250.00
AT505DC
Dichroic beamsplitters (mirrors),Wavelength (CWL): 505 nm,Type :BS,Prices do not include any shipping, tax or customs clearance charges 		4-6 Weeks $175.00
T515lp
Dichroic beamsplitters (mirrors),Wavelength (CWL): 515 nm,Type :BS,Prices do not include any shipping, tax or customs clearance charges 		4-6 Weeks $250.00
T565lpxr
Dichroic beamsplitters (mirrors),Wavelength (CWL): 565 nm,Type :BS,Prices do not include any shipping, tax or customs clearance charges 		4-6 Weeks $250.00
AT600DC
Dichroic beamsplitters (mirrors),Wavelength (CWL): 600 nm,Type :BS,Prices do not include any shipping, tax or customs clearance charges 		4-6 Weeks $175.00

AT600DC - Parameter

T565lpxr - Parameter

T515lp - Parameter

AT505DC - Parameter

T455lp - Parameter

T400lp - Parameter

T556lpxr - Parameter

T425lpxr - Parameter

T647lpxr - Parameter

T612lpxr - Parameter

T610lpxr - Parameter

89085-3 Filter Set - Parameter

89084v2-2 Filter Set - Parameter

69015 Filter Set - Parameter

69014 Filter Set - Parameter

69013v2 Filter Set - Parameter

69011 Filter Set - Parameter

69008 Filter Set - Parameter

59033 Filter Set - Parameter

59012-1 Filter Set - Parameter

59011-1 Filter Set - Parameter

59010 Filter Set - Parameter

59002v2 Filter Set - Parameter

59001v2 Filter Set - Parameter

49009 Filter Set - Parameter

49307 Filter Set - Parameter

49311 Filter Set - Parameter

49310 Filter Set - Parameter

49306 Filter Set - Parameter

49309 Filter Set - Parameter

49305 Filter Set - Parameter

49304 Filter Set - Parameter

49313 Filter Set - Parameter

49312 Filter Set - Parameter

49308 Filter Set - Parameter

49303 Filter Set - Parameter

49302 Filter Set - Parameter

49301 Filter Set - Parameter

49028 Filter Set - Parameter

49000 Filter Set - Parameter

AT600DC - Download

T565lpxr - Download

T515lp - Download

AT505DC - Download

T455lp - Download

T400lp - Download

T556lpxr - Download

T425lpxr - Download

T647lpxr - Download

T612lpxr - Download

T610lpxr - Download

89085-3 Filter Set - Download

89084v2-2 Filter Set - Download

69015 Filter Set - Download

69014 Filter Set - Download

69013v2 Filter Set - Download

69011 Filter Set - Download

69008 Filter Set - Download

59033 Filter Set - Download

59012-1 Filter Set - Download

59011-1 Filter Set - Download

59010 Filter Set - Download

59002v2 Filter Set - Download

59001v2 Filter Set - Download

49009 Filter Set - Download

49307 Filter Set - Download

49311 Filter Set - Download

49310 Filter Set - Download

49306 Filter Set - Download

49309 Filter Set - Download

49305 Filter Set - Download

49304 Filter Set - Download

49313 Filter Set - Download

49312 Filter Set - Download

49308 Filter Set - Download

49303 Filter Set - Download

49302 Filter Set - Download

49301 Filter Set - Download

49028 Filter Set - Download

49000 Filter Set - Download

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