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Structured Illumination Microscope | SIMTRUM Photonics Sotre

Structured Illumination Microscope
SIMSCOP Structured Illumination Microscope

SIM Basic - the super-resolution microscopy system that addresses deep biological questions with ease.

Our goal is to make super-resolution accessible to all scientists to advance their research. For this reason, we developed DS, the first super-resolution module that is compatible with any existing upright or inverted microscope and can be used like a confocal microscope to facilitate access to super-resolved deep data of biological samples.



Product Brochure Link: 


Three imaging modalities in one setup


By choosing SIM Basic technology, you will be able to create a modular, expandable, and highly performant system, resulting in the creation of a truly enabling technology. The SIM Basic can be used both with SpinDisk SIM confocal system as well as independently as a Stand-Alone system for any microscope that has a camera port.




A single click to double confocal resolution


Through the using of a multi-spot structured illumination system, SIM Basic provides reliable, easy-to-use and affordable solutions to study sub-cellular structures with a XY resolution of 100nm without requiring any special sample preparation protocol.


Mouse primary microglia, alpha-tubulin 488, 100X, 1.45 NA



Mouse primary microgila, alpha-tubulin 488, 100X, 1.45NA, 100nm resolution




Three methods are available for obtaining deep data



Mouse kidney section. Wheat Germ 

Agglutinin (WGA, green) and phalloidin 

(red) markers, 25 Sil, 105 NA acquired

 with SpinDisk SIM SPinning disk system.

With SIM Basic, large confocal acquisitions

can be enhanced by adding a deeper level of

detail thanks to super-resolved optical 

sectioning with Z resolution of up to 300 nm.



Cleared mouse kidney section stained with 

Alexa Fluor 488 labeling blood vessels.

Z stack 76µm and 3D rendering.

It can be used with samples with thicknesses comparable to those used in confocal microscopy, giving super-resolved data over a depth of 50 um.


In this way, native heterogeneous complex samples can be investigated more thoroughly using routine preparation protocols.



A two-fold increase in spatial resolution can be obtained using both high magnification (60X, 100X) and low magnification (20X, 40X) objectives, thereby enabling the study of complex 3D models such as tissues, spheroids, organoids, and small organisms.


Hippocampal coronal slice from Thy1-GFP mouse brain;

20X dry 0.75 NA.


At any moment, you can find out more


In order to provide maximum flexibility in fluorophore choice and optimal multichannel imaging without spectral overlap, we have designed the instrument to operate across the entire wavelength spectrum from 400 to 750 nm. By utilizing the dual camera function of the SpinDisk SIM spinning disk system, the SIM Basic can simultaneously acquire multiple channels, resulting in faster acquisition times.

3D volume view of a mouse brain tissue section showing neurons with dendritic

spines(green), microglia (red), astrocytes (white) and DNA (cyan). Total volume 

acquired: 30μm. 60X, oil 1.4 NA


The SIM Basic high-speed acquisition modality allows for the capture of meaningful data at high resolution while minimizing light exposure and therefore. The risk of photo-toxicity. A delicate specimen can be explored using this functionality.

Through a temporal resolution of >10fps (1024 x 1024 px FOV)

deep specimens can be monitiored dynamically for cellular and subcellular changes.




Product specifications and Brochures

Product Brochure Link: 


SIMTRUM offers Structure Illumination Microscope Basic version, this system is able to work with our Spinning Disk Confocal Microscope to achieve better resolution ~100nm

ParametersSIM Basic

SpinDisk SI(Upgrade) 

Spin Disk System details refer to Here

FOV1024 x 1024 pixel (66 x 66 µm 100X | 333 x 333 µm 20X)1024 x 1024 pixel (66 x 66 µm 100X | 333 x 333 µm 20X)
ResolutionLateral Resolution (FWHM): ~100 nm (100X NA 1.45) 
Axial Resolution (FWHM): ~300 nm (100X NA 1.45)
Lateral Resolution (FWHM): ~100 nm (100X NA 1.45)
Axial Resolution (FWHM): ~300 nm (100X NA 1.45)
DeepSIM Acquisition Speed13fps (1024x1024px)113fps (1024x1024px)
Laser Spectral RangeExcitation: 400-750 nm; emission: 400-850 nmExcitation: 400-750 nm; emission: 400-850 nm
Objective Specifications-from 20X to 100X magnification range
-high numerical aperture (NA)
-plan apochromat correction
-from 20X to 100X magnification range
-high numerical aperture (NA)
-plan apochromat correction
Camera CompatibilityAny triggerable camera having 6.5 µm pixel sizeAny triggerable camera having 6.5 µm pixel size
Multi Cameras OptionSingle cameraDual camera option available*
Spinning Disk UpgradeStand-alone solution AAdd-on compatible with SpinDisK Advance
Imaging ModalitiesSuper-resolution | WidefieldSuper-resolution | Confocal spinning disk | Widefield
Upgradable Microscope ConfigurationUpright and inverted configurationsInverted configuration
SoftwareµManager /VisiView® / NIS Ele mentsµManager /VisiView® / NIS Elem ents
Installation ConditionsTemperature 23 ± 5°C, Humidity 70% RH or lessTemperature 23 ± 5°C, Humidity 70% RH or less
Weight50.7 lbs | 23Kg44 lbs | 20Kg
Dimensions13.8 (w) x 20.2 (L) x 11.4 (h) inches
352.0 (w) 514.0 (L) x 290.5 (h) mm
14.0 (w) x 17.1 (L) x 11.4 (h) inches
356.0 (w) x 435.0 (L) x 290.5 (h) mm
Layout





In the past few years there has been a parallel evolution of technology and applications, resulting in an exponential growth of published papers about SR techniques or showing SR in life science applications.  However, despite the enormous potential of these techniques, there are still major limitations associated with SR, mostly related to its cost and complexity. Because of several negative connotations, including special sample preparation, poor depth penetration and prohibitive cost, SR is not yet routine in everyday laboratory life.

For these reasons, in SIMTRUM we are working to make SR accessible to all researchers and to advance the potential of their scientific discoveries, moving them to a  next level.

Our newest product, the SIM Basic, was developed with this purpose and it is the first SR module based on structured illumination  compatible with any existing upright or  inverted microscope with a camera port, it  is as easy to use as a CF microscope and it  enables scientists to access deep data about  their biological samples.

In particular, based on a multi-spot structured illumination system, the SIM Basic is able to study cellular structures up to an XY resolution of ~100 nm and a super-resolved  optical sectioning, with Z resolution up to ~300 nm, can be obtained using both high  (60X-100X) and low (20X-40X) magnification  objectives.


The following is our deconstruction of SIM Basic imaging capabilities for several different applications.



Application 1  Structured Illumination Microscopy with Low Magnification Objectives


Application 2   Live-cells Detatils and Dynamics with Structured Illumination Microscopy


Application 3   Deep Structured Illumination Microscopy of Neurobiological Samples


Application 4   Visualization of the Binding Pattern of Monoclonal Antibodies Against Klebsiella Pneumoniae









Parameters

Basic

P Series Box

Basic

P Series

Advance

P Series

Basic

L Series

Advance

L Series

SpinDisk

Basic

SpinDisk

 Advance

SIM BasicSpinDisk SIM
Image Frame Rate4fps@512x5124fps@512x5124fps@512x51210fps@1024x102414fps@1024x1024100fps@2048x2048100fps@2048x204813fps@1024x102413fps@1024x1024
Resolution~230 nm ~230 nm ~230 nm ~230 nm 150-200nm~230 nm ~230 nm ~100 nm ~100 nm 
Image Depth<100µm<100µm<100µm<100µm<600µm<200µm<200µm<50µm<200µm
No. of Laser 114144444
Wavelength Choice 

405/445/488

/525/561/640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/488/

525/561/640(nm)

405/488/561/

640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

405/445/470/520/

528/555/640(nm)

DetectorsDetectorDetectorDetectorCMOS CamerasCMOS CamerasCMOS CameraDual sCMOS Camera(Single sCMOS optional)sCMOS CameraDual sCMOS Camera(Single sCMOS optional)
Microscope NonInverted (Upright Customizeable)Inverted (Upright Customizeable)Inverted (Upright Customizeable)Inverted (Upright Customizeable)

Inverted or

Upright 

Inverted or

Upright 

Inverted or

Upright 

Inverted
StagesNon

Z Motorized

XY Manual

XYZ Motorized Z Motorized
XY Manual
XYZ Motorized XYZ Motorized XYZ Motorized XYZ Motorized XYZ Motorized 
Electronics system

Motorized Filter
Motorized Pin hole

Motorized FilterMotorized FilterMotorized FilterMotorized FilterMotorized Filter
image contrastMediumMediumHighMediumHighHighHighHighHigh
Upgrade optionNonNonUpgrade to 30fps high Speed NonNonNonUpgrade to SPIN Disk SIM 100nm resolutionUpgrade to SPIN Disk SIM 100nm resolutionNon
Customized Option

Yes
YesYesYes

ApplicationsBiomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning;Biomedicine: monochromatic fluorescence microscopic   observation of cells, 3D scanning;
Biomedicine: three-dimensional fluorescence microscopy imaging observation of cells and tissues, multi-channel fluorescence detection
Reflective 3D microscopic imaging, surface shape detection,   rapid analysis and measurement of materials and microelectronic device   surface shapeFast 3D imaging of thick tissue, 3D histopathological image   detection, 3D histomorphological research of small animal tissues and organs   such as brain neurons, liver, kidney, etc.Dynamic fluorescence imaging of living cells/tissues,   microscopic observation of small animals such as zebrafish and nematodesDynamic fluorescence imaging of living cells/tissues,   microscopic in vivo observation of small animals such as zebrafish and   nematodes, and in vivo observation of multi-channel fluorescent organelles.Dynamic fluorescence super-resolution imaging of living cells   can be used for live cell dynamics, allowing tracking of biological changes   at cellular and subcellular levelsDynamic fluorescence super-resolution imaging of living cells,   3D imaging of tissues, organoids, spheroids and small organisms.



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Compare Model Drawings & Specs Availability Reference Price
(USD)
SIM Basic
Structured Illumination Microscope, SIM Basic, Image frame rate 13fps@1024x1024, Resolution ~100nm, image depth <50um, No. of Laser - 4, Wavelength choice 405/445/470/520/528/555/640nm, sCMOS Camera detectors, Inverted or upright microscope, XYZ motorized stages, Motorized filter electronics system, high image contrast, upgrade to SpinDisk SIM 100nm resolution
6-10 Weeks Request for quote
SpinDisk SIM
Structured Illumination Microscope, SIM Basic, Image frame rate 13fps@1024x1024, Resolution ~100nm, image depth <50um, No. of Laser - 4, Wavelength choice 405/445/470/520/528/555/640nm, sCMOS Camera detectors, Inverted or upright microscope, XYZ motorized stages, Motorized filter electronics system, high image contrast, upgrade to SpinDisk SIM 100nm resolution
6-10 Weeks Request for quote

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